Centro de Histocompatibilidade do Centro, Portugal.
Cell Immunol. 2010;264(1):97-103. doi: 10.1016/j.cellimm.2010.05.004. Epub 2010 May 11.
To compare frequency and functional activity of peripheral blood (PB) Th(c)17, Th(c)1 and Treg cells and the amount of type 2 cytokines mRNA we recruited SLE patients in active (n=15) and inactive disease (n=19) and healthy age- and gender-matched controls (n=15). The study of Th(c)17, Th(c)1 and Treg cells was done by flow cytometry and cytokine mRNA by real-time PCR. Compared to NC, SLE patients present an increased proportion of Th(c)17 cells, but with lower amounts of IL-17 per cell and also a decreased frequency of Treg, but with increased production of TGF-beta and FoxP3 mRNA. Iotan active compared to inactive SLE, there is a marked decreased in frequency of Th(c)1 cells, an increased production of type 2 cytokines mRNA and a distinct functional profile of Th(c)17 cells. Our findings suggest a functional disequilibrium of T-cell subsets in SLE which may contribute to the inflammatory process and disease pathogenesis.
为了比较外周血（PB）Th(c)17、Th(c)1 和 Treg 细胞的频率和功能活性以及 2 型细胞因子 mRNA 的水平，我们招募了处于活动期（n=15）和非活动期（n=19）的 SLE 患者以及年龄和性别匹配的健康对照者（n=15）。通过流式细胞术检测 Th(c)17、Th(c)1 和 Treg 细胞，实时 PCR 检测细胞因子 mRNA。与 NC 相比，SLE 患者 Th(c)17 细胞比例增加，但每个细胞 IL-17 的含量较低，Treg 细胞频率降低，但 TGF-β和 FoxP3 mRNA 的产生增加。与非活动期 SLE 相比，活动期 SLE 患者 Th(c)1 细胞频率明显降低，2 型细胞因子 mRNA 的产生增加，Th(c)17 细胞的功能特征明显。我们的研究结果提示 SLE 患者 T 细胞亚群存在功能失衡，可能导致炎症过程和疾病发病机制。
