Commodity Utilization Research Unit, Southern Regional Research Center, Agricultural Research Service, US Department of Agriculture, 1100 Robert E. Lee Blvd, New Orleans, LA 70124, USA.
Phytomedicine. 2010 Nov;17(13):1027-32. doi: 10.1016/j.phymed.2010.03.023.
Cinnamon extracts (CE) are reported to have beneficial effects on people with normal and impaired glucose tolerance, the metabolic syndrome, type 2 diabetes, and insulin resistance. However, clinical results are controversial. Molecular characterization of CE effects is limited. This study investigated the effects of CE on gene expression in cultured mouse adipocytes. Water-soluble CE was prepared from ground cinnamon (Cinnamomum burmannii). Quantitative real-time PCR was used to investigate CE effects on the expression of genes coding for adipokines, glucose transporter (GLUT) family, and insulin-signaling components in mouse 3T3-L1 adipocytes. CE (100 μg/ml) increased GLUT1 mRNA levels 1.91±0.15, 4.39±0.78, and 6.98±2.18-fold of the control after 2-, 4-, and 16-h treatments, respectively. CE decreased the expression of further genes encoding insulin-signaling pathway proteins including GSK3B, IGF1R, IGF2R, and PIK3R1. This study indicates that CE regulates the expression of multiple genes in adipocytes and this regulation could contribute to the potential health benefits of CE.
肉桂提取物（CE）据报道对血糖正常和糖耐量受损、代谢综合征、2 型糖尿病和胰岛素抵抗的人有益。然而，临床结果存在争议。CE 作用的分子特征描述有限。本研究调查了 CE 对培养的小鼠脂肪细胞基因表达的影响。从肉桂（Cinnamomum burmannii）研磨物中制备水溶性 CE。采用实时定量 PCR 技术研究 CE 对编码脂肪因子、葡萄糖转运蛋白（GLUT）家族和胰岛素信号成分的基因在小鼠 3T3-L1 脂肪细胞中的表达的影响。CE（100μg/ml）分别在 2、4 和 16 小时处理后使 GLUT1 mRNA 水平增加 1.91±0.15、4.39±0.78 和 6.98±2.18 倍。CE 还降低了其他编码胰岛素信号通路蛋白的基因的表达，包括 GSK3B、IGF1R、IGF2R 和 PIK3R1。本研究表明，CE 调节脂肪细胞中多个基因的表达，这种调控可能有助于 CE 的潜在健康益处。
