Juengwatanatrakul Thaweesak, Jitsaeng Kusuma, Kaewamatawong Rawiwun
Faculty of Pharmaceutical Sciences, Ubon Ratchathani University, Warin Chamrap, Ubon Ratchathani, Thailand.
J Evid Based Integr Med. 2025 Jan-Dec;30:2515690X251324810. doi: 10.1177/2515690X251324810.
Diabetes mellitus, diagnosed as Madhumeho (sweet urine) in Thai traditional medicine, is believed to stem from imbalanced life elements. Ya That Opchoei mixture (YTO), a polyherbal mixture, is used to treat its symptoms. This study assessed the hypoglycemic potential of YTO and conducted qualitative and quantitative analyses of its bioactive constituents. YTO significantly inhibited -glucosidase at IC 0.05 ± 0.00 mg/mL and -amylase activities at IC 0.04±mg/mL. YTO at concentrations of 2.22 mg/mL significantly increased C-peptide secretion and stimulated glucose uptake. Liquid chromatography-mass spectrometry revealed constituents from , , , , and . High-performance liquid chromatography enabled quantification of bioactive compounds, including glycyrrhizic acid, eugenol, cinnamic acid, cinnamaldehyde, and coumarin. The coumarin content in 100 mL YTO was within the tolerable daily intake set by the European Food Safety Authority. This study confirms the hypoglycemic potential of YTO and presents its quality control process for safety assurance.
糖尿病在泰国传统医学中被诊断为“Madhumeho”（甜尿症），据信源于生活元素失衡。Ya That Opchoei合剂（YTO）是一种多草药合剂，用于治疗其症状。本研究评估了YTO的降血糖潜力，并对其生物活性成分进行了定性和定量分析。YTO在IC 0.05±0.00 mg/mL时显著抑制α-葡萄糖苷酶，在IC 0.04±mg/mL时抑制α-淀粉酶活性。浓度为2.22 mg/mL的YTO显著增加C肽分泌并刺激葡萄糖摄取。液相色谱-质谱联用分析显示其含有来自[此处原文缺失具体植物名称]、[此处原文缺失具体植物名称]、[此处原文缺失具体植物名称]、[此处原文缺失具体植物名称]和[此处原文缺失具体植物名称]的成分。高效液相色谱法能够对包括甘草酸、丁香酚、肉桂酸、肉桂醛和香豆素在内的生物活性化合物进行定量分析。100 mL YTO中的香豆素含量在欧洲食品安全局设定的每日可耐受摄入量范围内。本研究证实了YTO的降血糖潜力，并介绍了其用于安全保证的质量控制过程。
