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本文引用的文献

1
Genetic diversity of transmission blocking vaccine candidate (Pvs25 and Pvs28) antigen in Plasmodium vivax clinical isolates from Iran.伊朗间日疟原虫临床分离株中传播阻断疫苗候选抗原（Pvs25和Pvs28）的遗传多样性
Acta Trop. 2009 Mar;109(3):176-80. doi: 10.1016/j.actatropica.2008.09.012. Epub 2008 Sep 30.
2
Heme oxygenase-1 modulates early inflammatory responses: evidence from the heme oxygenase-1-deficient mouse.血红素加氧酶-1调节早期炎症反应：来自血红素加氧酶-1缺陷小鼠的证据。
Am J Pathol. 2004 Sep;165(3):1045-53. doi: 10.1016/S0002-9440(10)63365-2.
3
Blocking of transmission to mosquitoes by antibody to Plasmodium vivax malaria vaccine candidates Pvs25 and Pvs28 despite antigenic polymorphism in field isolates.尽管野外分离株存在抗原多态性，但间日疟原虫疟疾疫苗候选物Pvs25和Pvs28的抗体仍可阻断向蚊子的传播。
Am J Trop Med Hyg. 2003 Nov;69(5):536-41.
4
Oral immunization with a recombinant malaria protein induces conformational antibodies and protects mice against lethal malaria.用重组疟疾蛋白进行口服免疫可诱导构象抗体并保护小鼠免受致命性疟疾的侵害。
Infect Immun. 2003 May;71(5):2356-64. doi: 10.1128/IAI.71.5.2356-2364.2003.
5
Detection of vivax malaria sporozoites naturally infected in Anopheline mosquitoes from endemic areas of northern parts of Gyeonggi-do (Province) in Korea.韩国京畿道北部疟疾流行区按蚊体内自然感染的间日疟原虫子孢子的检测。
Korean J Parasitol. 2002 Jun;40(2):75-81. doi: 10.3347/kjp.2002.40.2.75.
6
The ears of the hippopotamus: manifestations, determinants, and estimates of the malaria burden.河马的耳朵：疟疾负担的表现、决定因素及评估
Am J Trop Med Hyg. 2001 Jan-Feb;64(1-2 Suppl):1-11. doi: 10.4269/ajtmh.2001.64.1.
7
Antibodies to malaria vaccine candidates Pvs25 and Pvs28 completely block the ability of Plasmodium vivax to infect mosquitoes.针对疟疾疫苗候选物Pvs25和Pvs28的抗体完全阻断了间日疟原虫感染蚊子的能力。
Infect Immun. 2000 Dec;68(12):6618-23. doi: 10.1128/IAI.68.12.6618-6623.2000.
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Sequence diversity in rodent malaria of the Pfs28 ookinete surface antigen homologs.疟原虫Pfs28动合子表面抗原同源物在啮齿动物疟疾中的序列多样性。
Mol Biochem Parasitol. 2000 Oct;110(2):429-34. doi: 10.1016/s0166-6851(00)00285-1.
9
Sequence polymorphism in two novel Plasmodium vivax ookinete surface proteins, Pvs25 and Pvs28, that are malaria transmission-blocking vaccine candidates.两种新型间日疟原虫动合子表面蛋白Pvs25和Pvs28中的序列多态性，这两种蛋白是疟疾传播阻断疫苗的候选物。
Mol Med. 1998 Dec;4(12):772-82.
10
Efficacy of a food plant-based oral cholera toxin B subunit vaccine.一种基于食用植物的口服霍乱毒素B亚单位疫苗的疗效。
Nat Biotechnol. 1998 Mar;16(3):292-7. doi: 10.1038/nbt0398-292.

抗重组Pvs25单克隆抗体对中华按蚊子孢子发育的阻断作用

Blocking effect of a monoclonal antibody against recombinant Pvs25 on sporozoite development in Anopheles sinensis.

作者信息

Moon Sung-Ung, Kim Hyung-Hwan, Kim Tong-Soo, Choi Kyung-Mi, Oh Chang-Mi, Ahn Yong-Joo, Hwang Seo-Kyoung, Sohn Youngjoo, Shin E-Hyun, Kim Hyuck, Lee Hyeong-Woo

机构信息

Department of Pathology, University of Florida, 1600 SW Archer Road, Gainesville, FL 32610, USA.

出版信息

Clin Vaccine Immunol. 2010 Aug;17(8):1183-7. doi: 10.1128/CVI.00101-10. Epub 2010 Jun 16.

DOI:10.1128/CVI.00101-10
PMID:20554802
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2916255/
Abstract

To develop a vaccine to block the transmission of vivax malaria, the gene encoding the ookinete surface protein Pvs25 was cloned from a Korean malaria patient. The Pvs25 gene was 660 bp long, encoding 219 amino acids. It was subcloned into the expression vector pQE30 and expressed in Escherichia coli. The expressed recombinant protein, named rPvs25, showed a molecular mass of approximately 25 kDa by SDS-PAGE analysis. An anti-rPvs25 monoclonal antibody produced in BALB/c mice was able to inhibit sporozoite development in the mosquito Anopheles sinensis, which is known as the malaria transmission vector in the Republic of Korea. In addition, rPvs25 produced a relatively high antibody titer in BALB/c mice that lasted for more than 6 months. Based on these results, we suggest that recombinant Pvs25 could be a useful antigen in the development of a vaccine to prevent local malaria transmission in the Republic of Korea.

摘要

为研发一种阻断间日疟传播的疫苗，从一名韩国疟疾患者身上克隆了编码动合子表面蛋白Pvs25的基因。Pvs25基因长度为660 bp，编码219个氨基酸。将其亚克隆到表达载体pQE30中并在大肠杆菌中表达。通过SDS-PAGE分析，表达的重组蛋白rPvs25显示分子量约为25 kDa。在BALB/c小鼠中产生的抗rPvs25单克隆抗体能够抑制中华按蚊（韩国已知的疟疾传播媒介）中疟原虫的发育。此外，rPvs25在BALB/c小鼠中产生了相对较高的抗体滴度，且持续超过6个月。基于这些结果，我们认为重组Pvs25可能是研发预防韩国当地疟疾传播疫苗的一种有用抗原。