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与沙棘( Hippophae L. )种质资源群体干缩病抗性相关的 SRAP 标记。

Associations of SRAP markers with dried-shrink disease resistance in a germplasm collection of sea buckthorn (Hippophae L.).

机构信息

Key Laboratory of Biotechnology and Bioresources Utilization, Dalian Nationalities University, Dalian City, Liaoning, P.R. China.

出版信息

Genome. 2010 Jun;53(6):447-57. doi: 10.1139/g10-020.

Abstract

Sea buckthorn (Hippophae L.) is a woody, outcrossing dioecious pioneer plant, being widely planted as a new berry crop with rich nutritional and medicinal compounds. This long-juvenile and long-lived woody plant can be more difficult to cultivate than other crop plants. Dried-shrink disease (DSD) is a dangerous pathogen that destroys sea buckthorn and halts commercial production. We estimated variability of sequence-related amplified polymorphism (SRAP) markers in 77 accessions of 22 sea buckthorn cultivars to seek markers associated with DSD resistance and help to identify potential breeding cultivars. Seventeen SRAP primer combinations generated 289 bands, with a mean of 17 bands per primer combination. At a Dice coefficient of 0.852, the dendrogram generated with 191 polymorphic bands clustered 73 accessions of Hippophae rhamnoides into 2 groups and 4 accessions of Hippophae salicifolia into 1 group. Eleven SRAP markers (Me1-Em3(600), Me1-Em1(680), Me2-Em1(650), Me2-Em1(950), Me3-Em6(1300), Me2-Em6(320), Me2-Em6(400), Me1-Em2(600), Me1-Em1(1200), Me1-Em1(1700), Me2-Em2(250)) were significantly correlated with DSD resistance (P < 0.001). These markers provide a viable option for breeding programs that select lineages with DSD resistance, especially when no other genetic information, such as linkage maps and quantitative trait loci, are available.

摘要

沙棘(Hippophae L.)是一种木本、异交的先锋植物,作为一种营养丰富、药用化合物丰富的新型浆果作物而被广泛种植。这种长期处于幼年状态且寿命较长的木本植物比其他作物更难种植。干枯收缩病(DSD)是一种危险的病原体,它会破坏沙棘并阻止商业生产。我们估计了 22 个沙棘品种的 77 个品种的序列相关扩增多态性(SRAP)标记的变异性,以寻找与 DSD 抗性相关的标记,并帮助鉴定潜在的育种品种。17 个 SRAP 引物组合产生了 289 个条带,每个引物组合平均产生 17 个条带。在 Dice 系数为 0.852 时,使用 191 个多态性条带生成的聚类图将 73 个胡颓子属 rhamnoides 品种分为 2 组,4 个胡颓子属 salicifolia 品种分为 1 组。11 个 SRAP 标记(Me1-Em3(600)、Me1-Em1(680)、Me2-Em1(650)、Me2-Em1(950)、Me3-Em6(1300)、Me2-Em6(320)、Me2-Em6(400)、Me1-Em2(600)、Me1-Em1(1200)、Me1-Em1(1700)、Me2-Em2(250))与 DSD 抗性显著相关(P < 0.001)。这些标记为选择具有 DSD 抗性的品系的育种计划提供了可行的选择,特别是在没有其他遗传信息(如连锁图谱和数量性状位点)可用的情况下。

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