Huang L K, Jiang X Y, Huang Q T, Xiao Y F, Chen Z H, Zhang X Q, Miao J M, Yan H D
Department of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Ya'an, Sichuan, China.
College of Life Science and Technology, Southwest University for Nationalities, Chengdu, Sichuan, China.
Genet Mol Res. 2014 Dec 4;13(4):10142-9. doi: 10.4238/2014.December.4.8.
Sequence-related amplified polymorphism (SRAP) markers were used to analyze and estimate the genetic variability, level of diversity, and relationships among 20 cultivars and strains of annual ryegrass (Lolium multiflorum Lam.). Eighteen SRAP primer combinations generated 334 amplification bands, of which 298 were polymorphic. The polymorphism information content ranged from 0.4715 (me10 + em1) to 0.5000 (me5 + em7), with an average of 0.4921. The genetic similarity coefficient ranged from 0.4304 to 0.8529, and coefficients between 0.65 and 0.90 accounted for 90.00%. The cluster analysis separated the accessions into five groups partly according to their germplasm resource origins.
利用序列相关扩增多态性（SRAP）标记对20个一年生黑麦草（Lolium multiflorum Lam.）品种和品系的遗传变异性、多样性水平及亲缘关系进行分析和评估。18对SRAP引物组合共产生334条扩增带，其中298条具有多态性。多态性信息含量范围为0.4715（me10 + em1）至0.5000（me5 + em7），平均为0.4921。遗传相似系数范围为0.4304至0.8529，0.65至0.90之间的系数占90.00%。聚类分析部分地根据种质资源来源将材料分为五组。
