Zou Nian-Li, Zhao Fang-Fang, Wang Yuan-Ping, Liu Ping, Cao San-Jie, Wen Xin-Tian, Huang Yong
Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, Sichuan, China.
Virus Genes. 2010 Oct;41(2):202-9. doi: 10.1007/s11262-010-0500-9. Epub 2010 Jun 17.
Between 2008 and 2010, 19 strains of infectious bronchitis virus (IBV) were isolated from the vaccinated chicken flocks in Sichuan province, China. The S1 genes of the isolates were amplified and sequenced. Phylogenetic analysis revealed that the 19 isolates and 37 reference IBV strains can be grouped into eight genotypes. Although IBVs of Taiwan-I type, massachusetts type, and proventriculitis type were isolated, but most isolates were LX4 genotype. Homology analysis of the sequences of S1 genes of the 19 isolates and 37 reference IBV strains revealed that the identity of the nucleotides and amino acid sequences of the S1 genes between the 15 LX4-type isolates and other IBV strains were 71.9-99.3% and 72.1-99.1%, respectively, while those of the analyzed IBV of LX4 type were 96.0-99.9% and 94.3-99.8%, respectively. The results from this study and other published results in the GenBank database showed that isolates circulating in Sichuan province in recent years were mainly LX4 genotype, which is the predominant genotype circulated in China in recent years.
2008年至2010年间，从中国四川省的免疫鸡群中分离出19株传染性支气管炎病毒（IBV）。对分离株的S1基因进行了扩增和测序。系统发育分析表明，这19株分离株和37株参考IBV毒株可分为8个基因型。虽然分离到了台湾-I型、马萨诸塞型和腺胃炎型的IBV，但大多数分离株为LX4基因型。对19株分离株和37株参考IBV毒株的S1基因序列进行同源性分析，结果显示15株LX4型分离株与其他IBV毒株之间S1基因核苷酸序列和氨基酸序列的同一性分别为71.9% - 99.3%和72.1% - 99.1%，而所分析的LX4型IBV之间的同一性分别为96.0% - 99.9%和94.3% - 99.8%。本研究结果以及GenBank数据库中其他已发表的结果表明，近年来在四川省流行的分离株主要为LX4基因型，这是近年来在中国流行的优势基因型。
