Naqi S A, Karaca K, Bauman B
Department of Avian and Aquatic Animal Medicine, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA.
Avian Pathol. 1993 Sep;22(3):555-64. doi: 10.1080/03079459308418943.
An antigen-capture enzyme-linked immunosorbent assay (C-ELISA) was developed for detection and identification of infectious bronchitis virus (IBV) serotypes Arkansas, Connecticut, and Massachusetts using monoclonal antibodies (MAbs) specific to the S1 glycoprotein of the respective serotype. The assay (designed as a double-antibody sandwich assay) gave the best results when the S1-specific MAb, antigen, and chicken serum were of the same serotype. However, when a group-specific (M glycoprotein-specific) MAb was used for antigen capture, a distinctive pattern of cross-reactivity was observed between the antigens and heterologous chicken sera, suggesting a complex distribution of epitopes on the IBV M glycoproteins. Treatment of antigen with NP40 enhanced the ELISA signal only when the M glycoprotein-specific MAb was used for antigen capture. Although C-ELISA was inconsistent in detecting IBV in chicken tissue homogenates, it was highly effective in detecting the virus in allantoic fluid after the homogenates were given one chicken embryo passage.
开发了一种抗原捕获酶联免疫吸附测定法（C-ELISA），用于使用针对各血清型S1糖蛋白的单克隆抗体（MAb）检测和鉴定传染性支气管炎病毒（IBV）的阿肯色州、康涅狄格州和马萨诸塞州血清型。该测定法（设计为双抗体夹心测定法）在S1特异性单克隆抗体、抗原和鸡血清为同一血清型时效果最佳。然而，当使用组特异性（M糖蛋白特异性）单克隆抗体进行抗原捕获时，观察到抗原与异源鸡血清之间存在独特的交叉反应模式，这表明IBV M糖蛋白上的表位分布复杂。仅当使用M糖蛋白特异性单克隆抗体进行抗原捕获时，用NP40处理抗原才会增强ELISA信号。尽管C-ELISA在检测鸡组织匀浆中的IBV时不一致，但在匀浆经过一次鸡胚传代后，它在检测尿囊液中的病毒方面非常有效。
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