Electron Microscope Unit, University of Maastricht, 6200 MD Maastricht, The Netherlands.
World J Gastroenterol. 2010 Jun 21;16(23):2851-66. doi: 10.3748/wjg.v16.i23.2851.
For an electron microscopic study of the liver, expertise and complicated, time-consuming processing of hepatic tissues and cells is needed. The interpretation of electron microscopy (EM) images requires knowledge of the liver fine structure and experience with the numerous artifacts in fixation, embedding, sectioning, contrast staining and microscopic imaging. Hence, the aim of this paper is to present a detailed summary of different methods for the preparation of hepatic cells and tissue, for the purpose of preserving long-standing expertise and to encourage new investigators and clinicians to include EM studies of liver cells and tissue in their projects.
为了进行肝脏的电子显微镜研究，需要专业知识和复杂、耗时的肝脏组织和细胞处理。电子显微镜（EM）图像的解释需要了解肝脏的精细结构以及在固定、嵌入、切片、对比染色和显微镜成像过程中遇到的各种伪影。因此，本文的目的是详细总结不同的肝脏细胞和组织制备方法，以保存长期以来的专业知识，并鼓励新的研究人员和临床医生在其项目中纳入对肝脏细胞和组织的 EM 研究。
