Numaga J, Matsuki K, Tokunaga K, Juji T, Mochizuki M
Department of Ophthalmology, Tokyo University School of Medicine, Japan.
Invest Ophthalmol Vis Sci. 1991 Jun;32(7):1958-61.
Vogt-Koyanagi-Harada syndrome (VKH) is a systemic disorder involving many organ systems, including the eye, the ear, the skin, and the meninges. One of the important clinical features of the disease is its strong association with histocompatibility antigens HLA-DR4 and HLA-Dw15. To investigate this association at the DNA level, the polymerase chain reaction technique was used. The DR beta 1 DNA sequence was analyzed to determine which part played the most significant role in the disease process. Although the number of patients studied was small, these data showed that these probes were useful in investigating the HLA-DR subtypes related to VKH. All but one patient (19 of 20) had a specific sequence encoding amino acids 70 and 71 of the HLA-DR beta 1 molecule. The data suggest that the sequence that encodes for amino acids 70 and 71 and adjacent ones of the HLA-DR beta 1 molecule could contribute to disease susceptibility.
伏格特-小柳-原田综合征（VKH）是一种累及多个器官系统的全身性疾病，包括眼睛、耳朵、皮肤和脑膜。该疾病的重要临床特征之一是与组织相容性抗原HLA-DR4和HLA-Dw15密切相关。为了在DNA水平上研究这种关联，采用了聚合酶链反应技术。对DRβ1 DNA序列进行分析，以确定哪一部分在疾病过程中起最重要作用。尽管研究的患者数量较少，但这些数据表明这些探针在研究与VKH相关的HLA-DR亚型方面很有用。除一名患者外（20例中的19例），所有患者都有一个编码HLA-DRβ1分子第70和71位氨基酸的特定序列。数据表明，编码HLA-DRβ1分子第70和71位氨基酸及其相邻氨基酸的序列可能与疾病易感性有关。
