Weinstein B I, Kandalaft N, Ritch R, Camras C B, Morris D J, Latif S A, Vecsei P, Vittek J, Gordon G G, Southren A L
Department of Ophthalmology, New York Medical College, Valhalla 10595.
Invest Ophthalmol Vis Sci. 1991 Jun;32(7):2130-5.
Glucocorticoids have long been implicated in the etiology of primary open-angle glaucoma (POAG) and cataract. Cortisol metabolites have biologic activity and may affect aqueous humor dynamics. This study was done to determine whether these metabolites are found in human aqueous humor and can be produced by ocular tissues. Radioimmunoassays (RIA) were developed for 5 alpha-dihydrocortisol (5 alpha-DHF) and 5 beta-dihydrocortisol (5 beta-DHF). These assays, as well as a cortisol RIA, were used to quantify these three steroids in 20 surgically derived aqueous humor specimens from patients with and without POAG. The mean concentrations of cortisol and 5 alpha-DHF were 2.5 and 1.3 ng/ml, respectively. In the small group studied, there was no statistically significant difference between the aqueous humor steroid levels in patients with and without POAG. The amount of 5 beta-DHF was at the lower limits of detection of the assay system and could not be uniquivocally shown. Human lenses metabolized cortisol in vitro to 5 alpha-DHF and 3 alpha,5 alpha-tetrahydrocortisol (3 alpha,5 alpha-THF). There was no 5 beta-DHF or cortisone formed. The 5 alpha-DHF and 3 alpha,5 alpha-THF were identified by their positions on thin-layer chromatography, their retention times on high-performance liquid chromatography, and recrystallization with authentic standards to constant specific activity. The data suggest that the lens is the source of 5 alpha-DHF in aqueous humor.
长期以来，糖皮质激素一直被认为与原发性开角型青光眼（POAG）和白内障的病因有关。皮质醇代谢产物具有生物活性，可能会影响房水动力学。本研究旨在确定这些代谢产物是否存在于人体房水中以及是否可由眼组织产生。针对5α-双氢皮质醇（5α-DHF）和5β-双氢皮质醇（5β-DHF）开发了放射免疫测定法（RIA）。这些测定法以及皮质醇RIA被用于对20份手术获取的房水标本中的这三种类固醇进行定量分析，这些标本来自患有和未患有POAG的患者。皮质醇和5α-DHF的平均浓度分别为2.5和1.3 ng/ml。在该小样本研究中，患有和未患有POAG的患者房水类固醇水平之间无统计学显著差异。5β-DHF的量处于检测系统的检测下限，无法明确显示。人晶状体在体外将皮质醇代谢为5α-DHF和3α,5α-四氢皮质醇（3α,5α-THF）。未形成5β-DHF或可的松。通过它们在薄层色谱上的位置、在高效液相色谱上的保留时间以及与标准品重结晶至恒定比活性来鉴定5α-DHF和3α,5α-THF。数据表明晶状体是房水中5α-DHF的来源。
