el-Barrawy M A
J Egypt Public Health Assoc. 1989;64(5-6):547-59.
The serological determination of class II antigens is still a mandatory test prior to allotransplantation. It is known that these antigens are normally expressed on B-lymphocytes and monocytes. The B-lymphocytes that constitute 10-15% of total blood lymphocytes are the cells currently used for HLA-DR typing. To avoid HLA-DR typing difficulties, or even impossibilities that are frequently encountered among some patient groups, we studied the response of peripheral blood mononuclear cells (PBMNC)--as an alternative source of cells for class II antigen typing--to in vitro mitogen and interleukin-2 activation and propagation. Although the patients included in this study were selected having pre-known HLA-DR typing difficulties, all of them could be adequately typed by this method.
在进行同种异体移植之前，血清学检测II类抗原仍然是一项强制性检查。众所周知，这些抗原通常在B淋巴细胞和单核细胞上表达。占血液淋巴细胞总数10%-15%的B淋巴细胞是目前用于HLA-DR分型的细胞。为避免在某些患者群体中经常遇到的HLA-DR分型困难甚至无法分型的情况，我们研究了外周血单个核细胞（PBMNC）——作为II类抗原分型的替代细胞来源——对体外有丝分裂原和白细胞介素-2激活及增殖的反应。尽管本研究纳入的患者是因已知存在HLA-DR分型困难而入选的，但所有患者均可通过该方法进行充分分型。
