Department of Pathology, School of Basic Medical Science, Wuhan University, Wuhan, China.
Eur J Histochem. 2010 Apr 14;54(2):e20. doi: 10.4081/ejh.2010.e20.
Quantum dots (QDs) are a new class of fluorescent probes to detect biomarker expression. The role of caveolin-1 (Cav-1) in tongue squamous cell carcinoma (TSCC) is still unknown. This study aimed to investigate the expression profile of Cav-1 in carcinogenesis and development of TSCC by QDs immunofluorescence histochemistry (QDs-IHC) and discuss the relationship between the Cav-1 expression and the clinicopathological outcomes. QDs-IHC was used to detect Cav-1 expression in tissue microarrays including normal tongue mucosa (NTM; n=10), hyperplastic tongue mucosa (HTM; n=10), tongue pre-cancer lesions (TPL; n=15) and primary tongue squamous cell carcinoma (PTSCC; n=61). Correlations between the Cav-1 expression and clinicopathologic variables were evaluated statistically. Cells positive for Cav-1 were clearly detected and bright images were obtained in a fine, granular pattern at the cell membrane and cytoplasm using QDs-IHC. The rate of Cav-1 immunoreactivity increased progressively from NTM (0%), HTM (0%), TPL (36%) to PTSCC (74%). When compared with each other, there was statistical significance among PTSCC, TPL and NTM as well as among PTSCC, TPL and HTM. Moreover, Cav-1 expression level in PTSCC was correlated positively with clinical stage and histologic grade. QDs-IHC could accurately detect protein location in tongue mucosa. An increased expression of Cav-1 in the stepwise carcinogenesis from NTM, HTM, TPL to PTSCC suggested that Cav-1 might be an oncogene in the development of tongue squamous cell carcinoma.
量子点 (QDs) 是一种新型荧光探针，用于检测生物标志物的表达。窖蛋白-1 (Cav-1) 在舌鳞状细胞癌 (TSCC) 中的作用尚不清楚。本研究旨在通过 QDs 免疫荧光组织化学 (QDs-IHC) 检测 Cav-1 在 TSCC 发生和发展过程中的表达谱，并探讨 Cav-1 表达与临床病理结局的关系。QDs-IHC 用于检测组织微阵列中 Cav-1 的表达，包括正常舌黏膜 (NTM; n=10)、增生性舌黏膜 (HTM; n=10)、舌前病变 (TPL; n=15) 和原发性舌鳞状细胞癌 (PTSCC; n=61)。统计评估 Cav-1 表达与临床病理变量之间的相关性。使用 QDs-IHC，细胞膜和细胞质中 Cav-1 阳性细胞清晰可见，呈细颗粒状明亮图像。Cav-1 免疫反应率从 NTM(0%)、HTM(0%)、TPL(36%)逐渐增加到 PTSCC(74%)。与彼此相比，PTSCC、TPL 和 NTM 之间以及 PTSCC、TPL 和 HTM 之间均有统计学意义。此外，PTSCC 中 Cav-1 的表达水平与临床分期和组织学分级呈正相关。QDs-IHC 可以准确检测舌黏膜中蛋白质的位置。Cav-1 在从 NTM、HTM、TPL 到 PTSCC 的逐步癌变过程中的表达增加表明，Cav-1 可能是舌鳞状细胞癌发生的癌基因。
