Department of Medical Microbiology, School of Medicine, University of Split, Šoltanska 2, Split, Croatia.
Department of Clinical Microbiology, University Hospital Split, Spinčićeva 1, 21000 Split, Croatia.
J Med Microbiol. 2010 Oct;59(Pt 10):1185-1190. doi: 10.1099/jmm.0.016964-0. Epub 2010 Jun 17.
The aim of this study was to determine the prevalence and antibiotic resistance rates of extended-spectrum β-lactamase (ESBL)-producing Proteus mirabilis strains isolated from inpatients at the Split University Hospital (southern Croatia) during a survey performed between 2005 and 2008. A total of 2152 consecutive isolates of P. mirabilis were isolated. The prevalence was 0.5 % in 2005 and increased significantly to 20.9 % by 2008. Strains were most frequently isolated from urine (36.5 %) and bronchial aspirates and wound swabs (11.3 %). ESBL-producing P. mirabilis isolates showed very high resistance rates to the majority of non-β-lactam antibiotics and were susceptible to a β-lactam/β-lactamase inhibitor and carbapenems. The isolates were genotyped and their ESBLs were molecularly characterized. Strains originating from the intensive care unit and the surgery and neurosurgery wards were clonally related. All P. mirabilis isolates produced the TEM-52 type of ESBL. To the best of our knowledge, our work detailed here and summarized in an earlier communication is the first report of such isolates from southern Croatia. Increased monitoring and screening for ESBL production in this species at our hospital is mandatory.
本研究旨在确定 2005 年至 2008 年期间在克罗地亚南部斯普利特大学医院住院患者中分离的产超广谱β-内酰胺酶（ESBL）的奇异变形杆菌的流行率和抗生素耐药率。共分离出 2152 株连续奇异变形杆菌分离株。2005 年的流行率为 0.5%，到 2008 年显著增加到 20.9%。该菌株最常从尿液（36.5%）和支气管吸出物和伤口拭子（11.3%）中分离出来。产 ESBL 的奇异变形杆菌分离株对大多数非β-内酰胺类抗生素表现出很高的耐药率，对β-内酰胺/β-内酰胺酶抑制剂和碳青霉烯类药物敏感。对分离株进行了基因分型，并对其 ESBL 进行了分子特征分析。来自重症监护病房和外科及神经外科病房的菌株具有克隆相关性。所有奇异变形杆菌分离株均产生 TEM-52 型 ESBL。据我们所知，我们在这里详细描述的工作以及之前的一份通讯中的总结是首次从克罗地亚南部报告的此类分离株。在我们医院对该物种中 ESBL 产生的监测和筛查是强制性的。
