Department of Gynecological Endocrinology and Fertility Disorders, Ruprechts-Karls University, Heidelberg, Germany.
Am J Reprod Immunol. 2010 Dec;64(6):402-10. doi: 10.1111/j.1600-0897.2010.00879.x.
Cytokines play a fundamental role at the feto-maternal interface. A dysregulation of the cytokine profile could be linked with fetal rejection. This study investigated differences in the expression of the Th1 cytokine interleukin-15 (IL-15) of normal and disturbed pregnancy.
Analysis of IL-15 expression by immunohistochemistry and real-time RT-PCR in placental tissue from normal pregnancies, spontaneous miscarriage and recurrent miscarriage (RM) was performed.
Expression of IL-15 was upregulated on mRNA and protein level in the placenta of miscarriage patients, especially in patients with RM. Immunohistochemistry and immunofluorescence double staining revealed extravillous trophoblast cells (EVT) as IL-15-expressing cells. Staining results were confirmed by real-time PCR (TaqMan).
Increased expression of IL-15 in the decidua of patients with RM may be linked to disturbed implantation and vascularization with consecutive placental and fetal rejection.
细胞因子在胎-母界面中起着至关重要的作用。细胞因子谱的失调可能与胎儿排斥有关。本研究旨在探讨正常妊娠和异常妊娠（如自然流产和复发性流产）中 Th1 细胞因子白细胞介素-15（IL-15）表达的差异。
通过免疫组织化学和实时 RT-PCR 分析正常妊娠、自然流产和复发性流产患者胎盘组织中 IL-15 的表达。
流产患者胎盘组织中 IL-15 的 mRNA 和蛋白水平均呈上调表达，尤其是复发性流产患者。免疫组织化学和免疫荧光双重染色显示，绒毛外滋养层细胞（EVT）是 IL-15 的表达细胞。实时 PCR（TaqMan）验证了染色结果。
复发性流产患者蜕膜中 IL-15 的表达增加可能与着床和血管化异常有关，进而导致胎盘和胎儿的排斥。
