Iizuka H, Adachi K, Halprin K M, Levine V
J Invest Dermatol. 1978 May;70(5):250-3. doi: 10.1111/1523-1747.ep12541392.
Using the uninvolved and involved skin from psoriatic patients, we investigated the effects of histamine and AMP (or adenosine) in vitro on the intracellular cyclic AMP levels. Both agents activated adenylate cyclase of the uninvolved and involved resulting in the accumulation of cyclic AMP. Without a cyclic nucleotide phosphodiesterase (PDE) inhibitor, these responses were biphasic and the maximal accumulation was observed in 5 min. With the PDE inhibitor both responses were markedly potentiated and high levels of cyclic AMP were observed for more than 20 min. The response to histamine by the involved skin was much greater than that by the uninvolved. The degree of the response to adenosine was approximately equal. In accordance with our previous work, the response to epinephrine by the involved skin was much less than that by the uninvolved. Thus adenylate cyclases of involved skin from psoriatic patients exhibit a markedly diminished response to epinephrine while at the same time exhibiting a markedly enhanced response to histamine. This precludes the possibility that the unresponsiveness to epinephrine can be due to a generalized inability of the epidermal psoriatic plaque cell to make a functioning cell membrane.
我们使用银屑病患者未受累及受累皮肤，在体外研究了组胺和AMP（或腺苷）对细胞内环磷酸腺苷（cAMP）水平的影响。这两种物质均激活了未受累及受累皮肤的腺苷酸环化酶，导致cAMP积累。在没有环核苷酸磷酸二酯酶（PDE）抑制剂的情况下，这些反应是双相的，在5分钟时观察到最大积累量。使用PDE抑制剂后，两种反应均明显增强，并且在20多分钟内观察到高水平的cAMP。受累皮肤对组胺的反应远大于未受累皮肤。对腺苷的反应程度大致相同。与我们之前的研究一致，受累皮肤对肾上腺素的反应远小于未受累皮肤。因此，银屑病患者受累皮肤的腺苷酸环化酶对肾上腺素的反应明显减弱，而同时对组胺的反应明显增强。这排除了对肾上腺素无反应可能是由于银屑病斑块表皮细胞普遍无法形成功能正常的细胞膜的可能性。
