猪皮肤（表皮）中的组胺（H2）受体 - 腺苷酸环化酶系统

Histamine (H2) receptor-adenylate cyclase system in pig skin (epidermis).

作者信息

Iizuka Hadachi K, Halprin K M, Levine V

出版信息

Biochim Biophys Acta. 1976 Jun 23;437(1):150-7. doi: 10.1016/0304-4165(76)90356-1.

Abstract

Histamine activated adenylate cyclase in pig skin (epidermal) slices, resulting in the accumulation of cyclic AMP. This effect was highly potentiated by the addition of cyclic AMP-phosphodiesterase inhibitors (theophylline, papaverine). A specific H2 receptor inhibitor (metiamide) inhibited the effect of histamine completely, while other antihistamines (diphenhydramine, acetophenazine, perphenazine, fluphenazine, promethazine) inhibited the effect of histamine to various lesser degrees. It has been shown that both epinephrine and prostaglandin E stimulate epidermal adenylate cyclase. Our data using specific blocking agents indicate that histamine, epinephrine and prostaglandin E2 act independently on the epidermal adenylate cyclase system.

摘要

组胺激活猪皮肤（表皮）切片中的腺苷酸环化酶，导致环磷酸腺苷（cAMP）积累。添加环磷酸腺苷磷酸二酯酶抑制剂（茶碱、罂粟碱）可显著增强这种效应。一种特异性H2受体抑制剂（甲硫米特）完全抑制组胺的作用，而其他抗组胺药（苯海拉明、乙酰奋乃静、奋乃静、氟奋乃静、异丙嗪）则不同程度地抑制组胺的作用。已表明肾上腺素和前列腺素E均可刺激表皮腺苷酸环化酶。我们使用特异性阻断剂的数据表明，组胺、肾上腺素和前列腺素E2对表皮腺苷酸环化酶系统的作用相互独立。