Adachi K, Iizuka H, Halprin K M, Levine V
Biochim Biophys Acta. 1977 Apr 27;497(2):428-36. doi: 10.1016/0304-4165(77)90200-8.
The cyclic AMP level in pig skin (epidermis) increases markedly after incubation with epinephrine, prostaglandin E, histamine or adenosine 5'-monophosphate. This increase is transient and "spiking" is the consistent response to these four stimulators. The "spiking" is due to a non-responsiveness or refractoriness which develops within minutes and is specific to any one stimulating hormone but not to the others. The addition of inhibitors of protein syntheses did not prevent the development of the refractoriness. Adenylate cyclase and phosphodiesterase activities measured in skin homogenates prepared from skin samples taken before, during and after the "spiking" did not change significantly. The hormone-induced refractoriness in this skin system appears to be due to a specific, localized loss of function of the adenylate cyclase system.
猪皮（表皮）在与肾上腺素、前列腺素E、组胺或5'-单磷酸腺苷孵育后，环磷酸腺苷水平显著升高。这种升高是短暂的，“峰值”是对这四种刺激物的一致反应。“峰值”是由于在几分钟内产生的无反应性或不应性，且这种无反应性或不应性对任何一种刺激激素具有特异性，对其他激素则不然。添加蛋白质合成抑制剂并不能阻止不应性的产生。在“峰值”出现之前、期间和之后采集的皮肤样本制备的皮肤匀浆中测得的腺苷酸环化酶和磷酸二酯酶活性没有显著变化。在这个皮肤系统中，激素诱导的不应性似乎是由于腺苷酸环化酶系统特定的、局部的功能丧失。
