REQUIMTE, CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, FCT, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
Int J Pharm. 2010 Aug 16;395(1-2):142-6. doi: 10.1016/j.ijpharm.2010.05.034. Epub 2010 May 26.
To test the possibility of obtain a material simultaneously biocompatible and microbiologically safe to be used as wound dressing material and as a matrix for drug release system, membranes with different initial contents in chitosan and 2-hydroxyethyl methacrylate (HEMA) have been prepared by gamma irradiation from a (60)Co source. The antimicrobial activity of obtained membranes against several reference strains was evaluated after inoculation. Sub-lethal gamma radiation doses were also applied in artificially contaminated membranes and the D(values) of microorganisms in use were determined in order to predict which radiation dose could guarantee membranes microbiological safety. In vitro haemolysis tests were also performed using drug loaded membranes irradiated at different doses. Results point out that those membranes naturally exhibit antimicrobial properties. Also show that, over the studied range values, drug loaded irradiated membranes display a non-significant level of haemolysis. These features show that the application of prepared membranes as a transdermal drug release system "ready to use" is viable.
为了测试获得一种同时具有生物相容性和微生物安全性的材料的可能性，该材料可用作伤口敷料材料和药物释放系统的基质，我们用γ射线从(60)Co 源辐照制备了具有不同初始壳聚糖和 2-羟乙基甲基丙烯酸酯（HEMA）含量的膜。在接种后，评估了获得的膜对几种参考菌株的抗菌活性。还在人工污染的膜上应用了亚致死γ辐射剂量，并确定了使用中的微生物的 D 值，以预测哪种辐射剂量可以保证膜的微生物安全性。还使用不同剂量辐照的载药膜进行了体外溶血试验。结果表明，这些膜天然具有抗菌性能。还表明，在所研究的范围内，载药辐照膜的溶血水平没有显著差异。这些特性表明，制备的膜作为“即用型”透皮药物释放系统的应用是可行的。
