Turner J N, Szarowski D H, Smith K L, Marko M, Leith A, Swann J W
Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509.
J Electron Microsc Tech. 1991 May;18(1):11-23. doi: 10.1002/jemt.1060180104.
Three-dimensional morphology and electrophysiology were correlated from individual neurons in a thick brain slice preparation. The hippocampal formation from immature and adult rats was cut transverse to the longitudinal axis into 500 microns-thick slices which were maintained under physiologic conditions. Individual neurons were impaled and physiologically characterized using microelectrodes. Recordings were made from the soma and in some cases from a dendrite. The impaled neurons were filled through the microelectrode with the fluorescent dye lucifer yellow and imaged by confocal scanning laser microscopy using an analog preprocessor. As many as 180 optical sections were recorded as a function of depth through the slices. Images are presented as a series of optical sections, stereo pairs, or three-dimensional reconstructions. Both stereo contouring and volume rendering methods were employed, and the reconstructions were viewed from any arbitrary perspective. Dendritic and axonal fields were separated from each other and displayed separately or as different pseudocolors. The three-dimensional reconstructions provided perspectives that were difficult or impossible to appreciate by viewing the optical sections or conventionally formed stereo pairs.
在厚脑片标本中，对单个神经元的三维形态和电生理学进行了关联研究。将幼年和成年大鼠的海马结构沿纵轴横向切成500微米厚的脑片，并维持在生理条件下。使用微电极刺入单个神经元并进行生理学特征分析。记录来自细胞体，在某些情况下还来自树突。通过微电极向刺入的神经元注入荧光染料荧光黄，并使用模拟预处理器通过共聚焦扫描激光显微镜成像。根据切片深度记录多达180个光学切片。图像以一系列光学切片、立体对或三维重建的形式呈现。采用了立体轮廓和体绘制方法，并且可以从任意角度查看重建结果。树突和轴突场相互分离，并分别显示或显示为不同的伪彩色。三维重建提供的视角是通过查看光学切片或传统形成的立体对难以或无法欣赏到的。
