• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

[18F]FLT PET 用于实验性脑胶质瘤基因治疗早期反应的无创监测。

[18F]FLT PET for non-invasive monitoring of early response to gene therapy in experimental gliomas.

机构信息

Laboratory for Gene Therapy and Molecular Imaging, Max Planck-Institute for Neurological Research, Gleuelerstr. 50, 50931, Cologne, Germany.

Center for Molecular Medicine (CMMC), Cologne, Germany.

出版信息

Mol Imaging Biol. 2011 Jun;13(3):547-557. doi: 10.1007/s11307-010-0361-6.

DOI:10.1007/s11307-010-0361-6
PMID:20563754
Abstract

The purpose of this study was to investigate the potential of 3'-deoxy-3'-[¹⁸F]fluorothymidine ([¹⁸F]FLT) positron emission tomography (PET) to detect early treatment responses in gliomas. Human glioma cells were stably transduced with genes yielding therapeutic activity, sorted for different levels of exogenous gene expression, and implanted subcutaneously into nude mice. Multimodality imaging during prodrug therapy included (a) magnetic resonance imaging, (b) PET with 9-(4-[¹⁸F]fluoro-3-hydroxymethylbutyl)guanine assessing exogenous gene expression, and (c) repeat [¹⁸F]FLT PET assessing antiproliferative therapeutic response. All stably transduced gliomas responded to therapy with significant reduction in tumor volume and [¹⁸F]FLT accumulation within 3 days after initiation of therapy. The change in [¹⁸F]FLT uptake before and after treatment correlated to volumetrically calculated growth rates. Therapeutic efficacy as monitored by [¹⁸F]FLT PET correlated to levels of therapeutic gene expression measured in vivo. Thus, [¹⁸F]FLT PET assesses early antiproliferative effects, making it a promising radiotracer for the development of novel treatments for glioma.

摘要

本研究旨在探讨 3'-去氧-3'-[¹⁸F]氟代胸苷([¹⁸F]FLT)正电子发射断层扫描(PET)在检测胶质瘤早期治疗反应中的潜力。人胶质瘤细胞被稳定转染产生治疗活性的基因，按不同水平的外源基因表达进行分选，并皮下植入裸鼠。在前药治疗期间进行的多模态成像包括：(a)磁共振成像，(b)评估外源基因表达的 9-(4-[¹⁸F]氟-3-羟甲基丁基)鸟嘌呤 PET，以及 (c)重复 [¹⁸F]FLT PET 评估抗增殖治疗反应。所有稳定转染的胶质瘤在治疗开始后 3 天内对治疗有反应，肿瘤体积和 [¹⁸F]FLT 积聚显著减少。治疗前后 [¹⁸F]FLT 摄取的变化与体积计算的生长率相关。通过 [¹⁸F]FLT PET 监测的治疗效果与体内测量的治疗基因表达水平相关。因此，[¹⁸F]FLT PET 评估早期抗增殖作用，使其成为开发新型胶质瘤治疗方法的有前途的示踪剂。

相似文献

1
[18F]FLT PET for non-invasive monitoring of early response to gene therapy in experimental gliomas.[18F]FLT PET 用于实验性脑胶质瘤基因治疗早期反应的无创监测。
Mol Imaging Biol. 2011 Jun;13(3):547-557. doi: 10.1007/s11307-010-0361-6.
2
Imaging-guided gene therapy of experimental gliomas.实验性胶质瘤的影像引导基因治疗
Cancer Res. 2007 Feb 15;67(4):1706-15. doi: 10.1158/0008-5472.CAN-06-2418.
3
Dynamic small-animal PET imaging of tumor proliferation with 3'-deoxy-3'-18F-fluorothymidine in a genetically engineered mouse model of high-grade gliomas.在高级别胶质瘤基因工程小鼠模型中，使用3'-脱氧-3'-18F-氟胸腺嘧啶核苷对肿瘤增殖进行动态小动物PET成像。
J Nucl Med. 2008 Mar;49(3):422-9. doi: 10.2967/jnumed.107.047092. Epub 2008 Feb 20.
4
Glioma proliferation as assessed by 3'-fluoro-3'-deoxy-L-thymidine positron emission tomography in patients with newly diagnosed high-grade glioma.通过3'-氟-3'-脱氧-L-胸腺嘧啶正电子发射断层扫描评估新诊断的高级别胶质瘤患者的胶质瘤增殖情况。
Clin Cancer Res. 2008 Apr 1;14(7):2049-55. doi: 10.1158/1078-0432.CCR-07-1553.
5
Early detection of response to experimental chemotherapeutic Top216 with [18F]FLT and [18F]FDG PET in human ovary cancer xenografts in mice.用 [18F]FLT 和 [18F]FDG PET 在荷人卵巢癌细胞移植瘤小鼠中早期检测对实验性化疗 Top216 的反应。
PLoS One. 2010 Sep 24;5(9):e12965. doi: 10.1371/journal.pone.0012965.
6
3'-deoxy-3'-[18F]fluorothymidine as a new marker for monitoring tumor response to antiproliferative therapy in vivo with positron emission tomography.3'-脱氧-3'-[18F]氟胸苷作为一种新型标志物，用于通过正电子发射断层扫描在体内监测肿瘤对增殖抑制治疗的反应。
Cancer Res. 2003 Jul 1;63(13):3791-8.
7
11C-methionine (MET) and 18F-fluorothymidine (FLT) PET in patients with newly diagnosed glioma.新诊断神经胶质瘤患者的11C-蛋氨酸（MET）和18F-氟代胸苷（FLT）正电子发射断层显像（PET）
Eur J Nucl Med Mol Imaging. 2008 Nov;35(11):2009-17. doi: 10.1007/s00259-008-0847-5. Epub 2008 Jun 10.
8
18F-fluoro-L-thymidine and 11C-methylmethionine as markers of increased transport and proliferation in brain tumors.18F-氟-L-胸腺嘧啶核苷和11C-甲基甲硫氨酸作为脑肿瘤中转运增加和增殖的标志物。
J Nucl Med. 2005 Dec;46(12):1948-58.
9
Gemcitabine Mechanism of Action Confounds Early Assessment of Treatment Response by 3'-Deoxy-3'-[18F]Fluorothymidine in Preclinical Models of Lung Cancer.在肺癌的临床前模型中，吉西他滨的作用机制使 3'-脱氧-3'-[18F]氟胸苷对治疗反应的早期评估产生混淆。
Cancer Res. 2016 Dec 15;76(24):7096-7105. doi: 10.1158/0008-5472.CAN-16-1479. Epub 2016 Oct 26.
10
[Correlation of 3'-deoxy-3'-18F-fluorothymidine uptake to cell proliferation in lung carcinoma xenografts].[3'-脱氧-3'-18F-氟胸苷摄取与肺癌异种移植瘤细胞增殖的相关性]
Ai Zheng. 2006 Dec;25(12):1512-6.

引用本文的文献

1
Potential F-RGD PET/CT and DCE-MRI Imaging-Based Biomarkers for Postoperative Survival Prediction Among Patients With Newly Diagnosed Glioblastoma Treated With Bevacizumab and Chemoradiotherapy.基于F-RGD PET/CT和DCE-MRI成像的潜在生物标志物用于预测接受贝伐单抗和放化疗的新诊断胶质母细胞瘤患者的术后生存率
Front Oncol. 2022 Aug 26;12:848266. doi: 10.3389/fonc.2022.848266. eCollection 2022.
2
Therapeutic Plasticity of Neural Stem Cells.神经干细胞的治疗可塑性
Front Neurol. 2020 Mar 20;11:148. doi: 10.3389/fneur.2020.00148. eCollection 2020.
3
Diagnostic and Predictive Value of Using RGD PET/CT in Patients with Cancer: A Systematic Review and Meta-Analysis.

本文引用的文献

1
Glioma proliferation as assessed by 3'-fluoro-3'-deoxy-L-thymidine positron emission tomography in patients with newly diagnosed high-grade glioma.通过3'-氟-3'-脱氧-L-胸腺嘧啶正电子发射断层扫描评估新诊断的高级别胶质瘤患者的胶质瘤增殖情况。
Clin Cancer Res. 2008 Apr 1;14(7):2049-55. doi: 10.1158/1078-0432.CCR-07-1553.
2
Dynamic small-animal PET imaging of tumor proliferation with 3'-deoxy-3'-18F-fluorothymidine in a genetically engineered mouse model of high-grade gliomas.在高级别胶质瘤基因工程小鼠模型中，使用3'-脱氧-3'-18F-氟胸腺嘧啶核苷对肿瘤增殖进行动态小动物PET成像。
J Nucl Med. 2008 Mar;49(3):422-9. doi: 10.2967/jnumed.107.047092. Epub 2008 Feb 20.
3
RGD PET/CT 在癌症患者中的诊断和预测价值：系统评价和荟萃分析。
Biomed Res Int. 2019 Jan 10;2019:8534761. doi: 10.1155/2019/8534761. eCollection 2019.
4
Stem Cell Tracking Technologies for Neurological Regenerative Medicine Purposes.用于神经再生医学目的的干细胞追踪技术
Stem Cells Int. 2017;2017:2934149. doi: 10.1155/2017/2934149. Epub 2017 Sep 12.
5
In vivo imaging of endogenous neural stem cells in the adult brain.成体脑内源性神经干细胞的体内成像。
World J Stem Cells. 2015 Jan 26;7(1):75-83. doi: 10.4252/wjsc.v7.i1.75.
6
A phase II trial of everolimus, temozolomide, and radiotherapy in patients with newly diagnosed glioblastoma: NCCTG N057K.依维莫司、替莫唑胺与放疗用于新诊断胶质母细胞瘤患者的II期试验：NCCTG N057K
Neuro Oncol. 2015 Sep;17(9):1261-9. doi: 10.1093/neuonc/nou328. Epub 2014 Dec 18.
7
Early assessment of the efficacy of temozolomide chemotherapy in experimental glioblastoma using [18F]FLT-PET imaging.使用 [18F]FLT-PET 成像技术早期评估替莫唑胺化疗治疗实验性脑胶质母细胞瘤的疗效。
PLoS One. 2013 Jul 4;8(7):e67911. doi: 10.1371/journal.pone.0067911. Print 2013.
8
New frontiers in the design and synthesis of imaging probes for PET oncology: current challenges and future directions.新型正电子发射断层扫描肿瘤成像探针设计与合成的前沿：当前挑战与未来方向
Mol Imaging Biol. 2012 Dec;14(6):653-66. doi: 10.1007/s11307-012-0590-y.
9
3'-deoxy-3'-18F-fluorothymidine PET and MRI for early survival predictions in patients with recurrent malignant glioma treated with bevacizumab.3'-去氧-3'-18F-氟代胸腺嘧啶 PET 和 MRI 对贝伐珠单抗治疗复发性恶性胶质瘤患者的早期生存预测
J Nucl Med. 2012 Jan;53(1):29-36. doi: 10.2967/jnumed.111.092387. Epub 2011 Dec 12.
10
Molecular imaging of gliomas with PET: opportunities and limitations.正电子发射断层扫描技术在脑胶质瘤中的分子影像学研究：机遇与挑战。
Neuro Oncol. 2011 Aug;13(8):806-19. doi: 10.1093/neuonc/nor054. Epub 2011 Jul 13.
Predicting treatment response of malignant gliomas to bevacizumab and irinotecan by imaging proliferation with [18F] fluorothymidine positron emission tomography: a pilot study.
通过[18F]氟代胸腺嘧啶正电子发射断层扫描成像增殖预测恶性胶质瘤对贝伐单抗和伊立替康的治疗反应：一项初步研究。
J Clin Oncol. 2007 Oct 20;25(30):4714-21. doi: 10.1200/JCO.2006.10.5825.
4
Kinetic analysis of 3'-deoxy-3'-18F-fluorothymidine in patients with gliomas.胶质瘤患者中3'-脱氧-3'-18F-氟胸苷的动力学分析。
J Nucl Med. 2006 Oct;47(10):1612-21.
5
18F-fluoro-L-thymidine and 11C-methylmethionine as markers of increased transport and proliferation in brain tumors.18F-氟-L-胸腺嘧啶核苷和11C-甲基甲硫氨酸作为脑肿瘤中转运增加和增殖的标志物。
J Nucl Med. 2005 Dec;46(12):1948-58.
6
Imaging proliferation in brain tumors with 18F-FLT PET: comparison with 18F-FDG.利用18F-FLT PET成像检测脑肿瘤增殖：与18F-FDG的比较。
J Nucl Med. 2005 Jun;46(6):945-52.
7
Early detection of tumor response to chemotherapy by 3'-deoxy-3'-[18F]fluorothymidine positron emission tomography: the effect of cisplatin on a fibrosarcoma tumor model in vivo.通过3'-脱氧-3'-[18F]氟胸苷正电子发射断层扫描早期检测肿瘤对化疗的反应：顺铂对体内纤维肉瘤肿瘤模型的影响。
Cancer Res. 2005 May 15;65(10):4202-10. doi: 10.1158/0008-5472.CAN-04-4008.
8
[18F]3'-deoxy-3'-fluorothymidine PET for the diagnosis and grading of brain tumors.[18F]3'-脱氧-3'-氟胸苷PET用于脑肿瘤的诊断与分级
Eur J Nucl Med Mol Imaging. 2005 Jun;32(6):653-9. doi: 10.1007/s00259-004-1742-3. Epub 2005 Feb 15.
9
Monitoring antiproliferative responses to kinase inhibitor therapy in mice with 3'-deoxy-3'-18F-fluorothymidine PET.用3'-脱氧-3'-¹⁸F-氟代胸苷PET监测小鼠对激酶抑制剂治疗的抗增殖反应。
J Nucl Med. 2005 Jan;46(1):114-20.
10
Imaging progress of herpes simplex virus type 1 thymidine kinase suicide gene therapy in living subjects with positron emission tomography.正电子发射断层扫描术对活体受试者进行单纯疱疹病毒I型胸苷激酶自杀基因治疗的成像进展
Cancer Gene Ther. 2005 Mar;12(3):329-39. doi: 10.1038/sj.cgt.7700795.