Department of Medicine, SUNY Upstate Medical University, Syracuse, New York 13210, USA.
J Cell Biochem. 2010 Jul 1;110(4):875-81. doi: 10.1002/jcb.22599.
TPM1kappa is an alternatively spliced isoform of the TPM1 gene whose specific role in cardiac development and disease is yet to be elucidated. Although mRNA studies have shown TPM1kappa expression in axolotl heart and skeletal muscle, it has not been quantified. Also the presence of TPM1kappa protein in axolotl heart and skeletal muscle has not been demonstrated. In this study, we quantified TPM1kappa mRNA expression in axolotl heart and skeletal muscle. Using a newly developed TPM1kappa specific antibody, we demonstrated the expression and incorporation of TPM1kappa protein in myofibrils of axolotl heart and skeletal muscle. The results support the potential role of TPM1kappa in myofibrillogenesis and sarcomeric function.
TPM1kappa 是 TPM1 基因的一种选择性剪接异构体，其在心脏发育和疾病中的具体作用尚待阐明。尽管 mRNA 研究表明 TPM1kappa 在蝾螈心脏和骨骼肌中有表达，但尚未对其进行定量。此外，也尚未证明 TPM1kappa 蛋白在蝾螈心脏和骨骼肌中的存在。在本研究中，我们对蝾螈心脏和骨骼肌中的 TPM1kappa mRNA 表达进行了定量。使用新开发的 TPM1kappa 特异性抗体，我们证明了 TPM1kappa 蛋白在蝾螈心脏和骨骼肌肌原纤维中的表达和掺入。这些结果支持了 TPM1kappa 在肌原纤维发生和肌节功能中的潜在作用。
