Institute of Functional Genomics, University of Regensburg, Regensburg, Germany.
Electrophoresis. 2010 Jul;31(14):2365-73. doi: 10.1002/elps.200900778.
Bovine serum, EDTA-plasma and EDTA-plasma fortified with acetylsalicylic acid (ASA) as antioxidant were compared with regard to their suitability for metabolomic studies. Metabolic fingerprints were generated from GC-TOF-MS data using the Leco ChromaTOF software in combination with the in-house retention time correction and data alignment tool INCA. A total of 6, 9 and 21 significant features with a false discovery rate of <0.05 were identified by INCA upon comparing EDTA- versus EDTA-ASA-plasma, EDTA-plasma versus serum and EDTA-ASA-plasma versus serum, respectively. To confirm that the observed signal intensities in the GC-TOF-MS fingerprints reflected true metabolite abundances, 19 amino acids, glucose and 6 organic acids were quantified by means of GC-MS using stable-isotope-labeled internal standards. As observed with the fingerprints, only the concentrations of lactate and citrate were found to be significantly lower in EDTA-plasma and serum, respectively, whereas the concentrations of the other metabolites were similar among the three sample types investigated.
比较了牛血清、EDTA 血浆和 EDTA 血浆中添加抗氧剂乙酰水杨酸（ASA）作为抗氧化剂，以评估它们在代谢组学研究中的适用性。使用 Leco ChromaTOF 软件，结合内部保留时间校正和数据对齐工具 INCA，从 GC-TOF-MS 数据生成代谢指纹图谱。通过 INCA 比较 EDTA-与 EDTA-ASA-血浆、EDTA-血浆与血清以及 EDTA-ASA-血浆与血清，分别鉴定出 6、9 和 21 个具有 <0.05 错误发现率的显著特征。为了确认 GC-TOF-MS 指纹图谱中观察到的信号强度反映了真实的代谢物丰度，使用 GC-MS 通过稳定同位素标记的内标定量测定了 19 种氨基酸、葡萄糖和 6 种有机酸。与指纹图谱观察到的一致，仅发现 EDTA-血浆和血清中乳酸盐和柠檬酸盐的浓度显著降低，而其他代谢物的浓度在三种研究的样本类型之间相似。
