鼠李糖乳杆菌 B 103 对木薯废水中 L(+)-乳酸生产的改善。
Improvement of L(+)-lactic acid production from cassava wastewater by Lactobacillus rhamnosus B 103.
机构信息
UNESP-Department of Biochemistry and Microbiology, Biological Sciences Institute, Universidade Estadual Paulista, Rio Claro, SP, Brazil.
出版信息
J Sci Food Agric. 2010 Aug 30;90(11):1944-50. doi: 10.1002/jsfa.4039.
BACKGROUND
L(+)-Lactic acid is used in the pharmaceutical, textile and food industries as well as in the synthesis of biodegradable plastics. The aim of this study was to investigate the effects of different medium components added in cassava wastewater for the production of L(+)-lactic acid by Lactobacillus rhamnosus B 103.
RESULTS
The use of cassava wastewater (50 g L(-1) of reducing sugar) with Tween 80 and corn steep liquor, at concentrations (v/v) of 1.27 mL L(-1) and 65.4 mL L(-1) respectively led to a lactic acid concentration of 41.65 g L(-1) after 48 h of fermentation. The maximum lactic acid concentration produced in the reactor after 36 h of fermentation was 39.00 g L(-1) using the same medium, but the pH was controlled by addition of 10 mol L(-1) NaOH.
CONCLUSION
The use of cassava wastewater for cultivation of L. rhamnosus is feasible, with a considerable production of lactic acid. Furthermore, it is an innovative proposal, as no references were found in the scientific literature on the use of this substrate for lactic acid production.
背景
L(+)-乳酸在制药、纺织和食品工业中以及可生物降解塑料的合成中都有使用。本研究旨在探讨在木薯废水中添加不同培养基成分对鼠李糖乳杆菌 B 103 生产 L(+)-乳酸的影响。
结果
使用木薯废水（还原糖浓度为 50 g/L），添加浓度分别为 1.27 mL/L 和 65.4 mL/L 的吐温 80 和玉米浆，发酵 48 小时后可得到 41.65 g/L 的乳酸浓度。在相同的培养基中，使用相同的培养基，在发酵 36 小时后在反应器中可产生最高 39.00 g/L 的乳酸，但通过添加 10 mol/L NaOH 来控制 pH 值。
结论
利用木薯废水培养鼠李糖乳杆菌是可行的，可大量生产乳酸。此外，这是一个创新性的建议，因为在科学文献中没有发现使用这种底物生产乳酸的参考文献。
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