Department of Pathology, School of Veterinary Medicine and Animal Sciences, University of São Paulo, São Paulo, SP, Brazil.
Phytother Res. 2011 Jan;25(1):11-6. doi: 10.1002/ptr.3216.
The objective of this work is to report the antiproliferative effect of P. cupana treatment in Ehrlich Ascites Carcinoma (EAC)-bearing animals. Female mice were treated with three doses of powdered P. cupana (100, 1000 and 2000 mg/kg) for 7 days, injected with 10(5) EAC cells and treated up to day 21. In addition, a survival experiment was carried out with the same protocol. P. cupana decreased the ascites volume (p = 0.0120), cell number (p = 0.0004) and hemorrhage (p = 0.0054). This occurred through a G1-phase arrest (p < 0.01) induced by a decreased gene expression of Cyclin D1 in EAC cells. Furthermore, P. cupana significantly increased the survival of EAC-bearing animals (p = 0.0012). In conclusion, the P. cupana growth control effect in this model was correlated with a decreased expression of cyclin D1 and a G1 phase arrest. These results reinforce the cancer therapeutic potential of this Brazilian plant.
本研究旨在报告蒲葵提取物对艾氏腹水癌（EAC）荷瘤动物的抗增殖作用。雌性小鼠连续 7 天用 3 种剂量的蒲葵粉（100、1000 和 2000mg/kg）处理，注射 10（5）个 EAC 细胞，并持续至第 21 天。此外，还进行了相同方案的生存实验。蒲葵提取物可降低腹水体积（p = 0.0120）、细胞数量（p = 0.0004）和出血（p = 0.0054）。这是通过 EAC 细胞中细胞周期蛋白 D1 基因表达的降低导致 G1 期阻滞（p < 0.01）实现的。此外，蒲葵提取物还显著提高了 EAC 荷瘤动物的存活率（p = 0.0012）。总之，在该模型中，蒲葵对肿瘤生长的控制作用与细胞周期蛋白 D1 的表达降低和 G1 期阻滞相关。这些结果强化了这种巴西植物的癌症治疗潜力。
