Carvalho Lidiane Vasconcelos do Nascimento, Cordeiro Marina Ferraz, E Lins Thiago Ubiratan Lins, Sampaio Maria Clara Pinheiro Duarte, de Mello Gabriela Souto Vieira, da Costa Valécia de Cassia Mendonça, Marques Leila Larisa Medeiros, Klein Traudi, de Mello João Carlos Palazzo, Cavalcanti Isabella Macário Ferro, Pitta Ivan da Rocha, Galdino da Rocha Pitta Maira, Rêgo Moacyr Jesus Barreto de Melo
Laboratory of Immunomodulation and New Therapeutical Approaches, Research Centre for Therapeutic Innovation Suely Galdino (NUPIT-SG), Federal University of Pernambuco, Recife, PE, Brazil.
Pharmacy Department, Maringa State University, Maringa, PR, Brazil.
Evid Based Complement Alternat Med. 2016;2016:1203274. doi: 10.1155/2016/1203274. Epub 2016 Dec 7.
(Guarana) is a native plant of Amazon region that has very traditional importance. Its seeds are rich in bioactive compounds, including tannins, which exhibit relevant properties. This study aimed to evaluate antibacterial, antineoplastic, and immunomodulatory activity of seeds crude extract (CE) and ethyl-acetate fraction (EAF). Antibacterial activity was evaluated by determination of minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). Antineoplastic activity was evaluated by MTT assays in hepatocellular carcinoma (HepG2), breast adenocarcinoma (MCF-7), ductal carcinoma (T47-D), non-Hodgkin's B cell lymphoma (Toledo), T cell leukemia (Jukart), and Acute Leukemia (HL-60) cell lines. BALB/c mice splenocytes were treated to assess IFN-, IL-6, IL-17, and IL-10 levels by sandwich ELISA. CE and EAF were not toxic to peripheral blood cells and splenocytes. CE and EAF fractions showed a bacteriostatic activity (MIC = 250 g/mL) and presented IC values of 70.25 g/mL and 61.18 g/mL in HL-60 leukemia cell line. All cytokines evaluated had their levels reduced after treatment, following dose-response model. Different biological activities were observed for both CE and EAF, suggesting as a source of bioactive substances, especially tannins that may be used for several diseases treatments.
瓜拉那是亚马逊地区的一种本土植物，具有非常重要的传统意义。其种子富含生物活性化合物，包括具有相关特性的单宁。本研究旨在评估种子粗提物（CE）和乙酸乙酯馏分（EAF）的抗菌、抗肿瘤和免疫调节活性。通过测定最低抑菌浓度（MIC）和最低杀菌浓度（MBC）评估抗菌活性。通过MTT法在肝癌细胞系（HepG2）、乳腺腺癌细胞系（MCF-7）、导管癌细胞系（T47-D）、非霍奇金B细胞淋巴瘤细胞系（Toledo）、T细胞白血病细胞系（Jukart）和急性白血病细胞系（HL-60）中评估抗肿瘤活性。通过夹心ELISA法处理BALB/c小鼠脾细胞以评估IFN-、IL-6、IL-17和IL-10水平。CE和EAF对外周血细胞和脾细胞无毒。CE和EAF馏分显示出抑菌活性（MIC = 250μg/mL），并且在HL-60白血病细胞系中的IC值分别为70.25μg/mL和61.18μg/mL。处理后，所有评估的细胞因子水平均按照剂量反应模型降低。CE和EAF均观察到不同的生物活性，表明其作为生物活性物质的来源，尤其是可用于多种疾病治疗的单宁。
