Department of Chemical Engineering, Industrial Chemistry and Material Science, University of Pisa, Via Diotisalvi 2, 56126 Pisa, Italy.
Biotechnol Bioeng. 2010 Aug 1;106(5):804-17. doi: 10.1002/bit.22740.
An innovative approach has been employed for the realization of bioactive scaffolds able to mimic the in vivo cellular microenvironment for tissue engineering applications. This method is based on the combination of molecular imprinting and soft-lithography technology to enhance cellular adhesion and to guide cell growth and proliferation due to presence of highly specific recognition sites of selected biomolecules on a well-defined polymeric microstructure. In this article polymethylmethacrylate (PMMA) scaffolds have been realized by using poly(dimethylsiloxane) (PDMS) microstructured molds imprinted with FITC-albumin and TRITC-lectin. In addition gelatin, an adhesion protein, was employed for the molecular imprinting of polymeric scaffolds for cellular tests. The most innovative aspect of this research was the molecular imprinting of whole cells for the development of substrates able to enhance the cell adhesion processes.
已经采用了一种创新的方法来实现具有生物活性的支架，以模拟体内细胞微环境，用于组织工程应用。该方法基于分子印迹和软光刻技术的结合，以增强细胞黏附，并由于在明确定义的聚合物微结构上存在所选生物分子的高度特异性识别位点，从而指导细胞生长和增殖。在本文中，通过使用印迹有 FITC-白蛋白和 TRITC-凝集素的聚二甲基硅氧烷 (PDMS) 微结构化模具，实现了聚甲基丙烯酸甲酯 (PMMA) 支架。此外，还使用了明胶（一种黏附蛋白）用于对用于细胞测试的聚合物支架进行分子印迹。这项研究的最具创新性的方面是对整个细胞进行分子印迹，以开发能够增强细胞黏附过程的基底。
