Liu Guangyang, Huang Xiaodong, Li Lingyun, Xu Xiaomin, Zhang Yanguo, Lv Jun, Xu Donghui
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Key Laboratory of Vegetables Quality and Safety Control, Laboratory of Quality & Safety Risk Assessment for vegetable Products, Ministry of Agriculture and Rural Affairs of China, Beijing 100081, China.
Nanomaterials (Basel). 2019 Jul 18;9(7):1030. doi: 10.3390/nano9071030.
Molecular imprinting technology (MIT), also known as molecular template technology, is a new technology involving material chemistry, polymer chemistry, biochemistry, and other multi-disciplinary approaches. This technology is used to realize the unique recognition ability of three-dimensional crosslinked polymers, called the molecularly imprinted polymers (MIPs). MIPs demonstrate a wide range of applicability, good plasticity, stability, and high selectivity, and their internal recognition sites can be selectively combined with template molecules to achieve selective recognition. A molecularly imprinted fluorescence sensor (MIFs) incorporates fluorescent materials (fluorescein or fluorescent nanoparticles) into a molecularly imprinted polymer synthesis system and transforms the binding sites between target molecules and molecularly imprinted materials into readable fluorescence signals. This sensor demonstrates the advantages of high sensitivity and selectivity of fluorescence detection. Molecularly imprinted materials demonstrate considerable research significance and broad application prospects. They are a research hotspot in the field of food and environment safety sensing analysis. In this study, the progress in the construction and application of MIFs was reviewed with emphasis on the preparation principle, detection methods, and molecular recognition mechanism. The applications of MIFs in food and environment safety detection in recent years were summarized, and the research trends and development prospects of MIFs were discussed.
分子印迹技术（MIT），也被称为分子模板技术，是一种涉及材料化学、高分子化学、生物化学等多学科方法的新技术。该技术用于实现三维交联聚合物（称为分子印迹聚合物，MIPs）的独特识别能力。MIPs具有广泛的适用性、良好的可塑性、稳定性和高选择性，其内部识别位点可与模板分子选择性结合以实现选择性识别。分子印迹荧光传感器（MIFs）将荧光材料（荧光素或荧光纳米颗粒）引入分子印迹聚合物合成体系，将目标分子与分子印迹材料之间的结合位点转化为可读取的荧光信号。该传感器展现出荧光检测高灵敏度和高选择性的优势。分子印迹材料具有重要的研究意义和广阔的应用前景。它们是食品和环境安全传感分析领域的研究热点。在本研究中，对MIFs的构建与应用进展进行了综述，重点阐述了其制备原理、检测方法及分子识别机制。总结了近年来MIFs在食品和环境安全检测中的应用，并探讨了MIFs的研究趋势和发展前景。
