神经生长因子高分子量形式（7S NGF）的结晶与表征

Crystallization and characterization of the high molecular weight form of nerve growth factor (7 S NGF).

作者信息

McDonald N Q, Blundell T L

机构信息

Department of Crystallography, Birkbeck College, London, U.K.

出版信息

J Mol Biol. 1991 Jun 20;219(4):595-601. doi: 10.1016/0022-2836(91)90655-p.

DOI:10.1016/0022-2836(91)90655-p

PMID:2056528

Abstract

A high molecular weight form of nerve growth factor (7 S NGF) has been crystallized in two crystal forms from polyethylene glycol 4000 by the vapour diffusion technique. The orthorhombic form A belongs to the space group P2(1)2(1)2(1) and has cell dimensions of a = 95.6, b = 96.5 and c = 147.0 A. With synchrotron X-ray radiation, these crystals diffract to 2.8 A resolution. They contain an intact 7 S NGF complex in the asymmetric unit. The tetragonal form B, which grows at similar conditions to the A form, belongs to the space group P4(1)2(1)2 (or P4(3)2(1)2) with unit cell dimensions of a = 97.4, b = 97.4 and c = 308.3 A. These crystals diffract to 3.6 A resolution and contain one 7 S complex per asymmetric unit. Native X-ray data have been collected to 3.3 A for the A form and to 5.0 A for the B form, both using synchrotron radiation.

摘要

一种高分子量形式的神经生长因子（7S NGF）已通过气相扩散技术从聚乙二醇4000中结晶出两种晶体形式。正交晶型A属于空间群P2(1)2(1)2(1)，晶胞参数为a = 95.6、b = 96.5和c = 147.0 Å。利用同步辐射X射线，这些晶体的衍射分辨率达到2.8 Å。它们在不对称单元中包含一个完整的7S NGF复合物。四方晶型B在与晶型A相似的条件下生长，属于空间群P4(1)2(1)2（或P4(3)2(1)2），晶胞参数为a = 97.4、b = 97.4和c = 308.3 Å。这些晶体的衍射分辨率达到3.6 Å，且每个不对称单元包含一个7S复合物。两种晶型均使用同步辐射收集了原生X射线数据，晶型A的数据收集到3.3 Å，晶型B的数据收集到5.0 Å。