神经营养因子-3的结晶及初步结构研究
Crystallization and preliminary structural studies of neurotrophin-3.
作者信息
Kelly J A, Singer E, Osslund T D, Yeates T O
机构信息
Molecular Biology Institute, University of California, Los Angeles 90024-1570.
出版信息
Protein Sci. 1994 Jun;3(6):982-3. doi: 10.1002/pro.5560030614.
Neurotrophin-3 (NT-3) has been crystallized in 2 forms. Orthorhombic crystals, space group P2(1)2(1)2, diffracted to 2.8 A and have cell dimensions a = 39.1 A, b = 54.0 A, and c = 65.5 A. The second form is space group P4(3)2(1)2, with cell dimensions a = b = 67.1 A, and c = 107.9 A. The tetragonal crystals diffract to 2.8 A at room temperature and 2.5 A at -100 degrees C. The unit cell dimensions change significantly upon freezing, a = b = 66.1 A, and c = 102.8 A. Phases for the orthorhombic form were obtained by molecular replacement using nerve growth factor as the search model. A partially refined model of the NT-3 dimer (75% complete) was then oriented and positioned in the tetragonal cell.
神经营养因子-3（NT-3）已形成两种晶体形式。正交晶体，空间群P2(1)2(1)2，衍射极限为2.8 Å，晶胞参数a = 39.1 Å，b = 54.0 Å，c = 65.5 Å。第二种形式是空间群P4(3)2(1)2，晶胞参数a = b = 67.1 Å，c = 107.9 Å。四方晶体在室温下衍射极限为2.8 Å，在-100℃时为2.5 Å。冷冻后晶胞参数发生显著变化，a = b = 66.1 Å，c = 102.8 Å。正交形式的相位通过以神经生长因子为搜索模型的分子置换获得。然后将NT-3二聚体的部分精修模型（完成75%）在四方晶胞中进行定向和定位。
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