Wheat Pathogenesis Programme, Plant-Pathogen Interactions Division, Rothamsted Research, Harpenden, Herts. AL5 2JQ, UK.
Mol Plant Pathol. 2005 Sep 1;6(5):527-40. doi: 10.1111/j.1364-3703.2005.00304.x.
SUMMARY Pathogenic microbes must successfully adapt to the host environment, acquiring nutrients and tolerating immune/defence responses. Studies on host-pathogen interactions at the transcriptome level have predominantly investigated host responses. Here we present a broad-scale transcriptional analysis on a fungal pathogen during sporulation within its host environment. Septoria leaf blotch is an important fungal disease of cultivated wheat and is caused by the ascomycete fungus Septoria tritici (teleomorph Mycosphaerella graminicola). A cDNA microarray containing 2563 unigenes was generated and then used to compare fungal nutrition and development in vitro under nutrient-rich and nutrient-limiting conditions and in vivo at a late stage of plant infection. The data obtained provided clear insights into metabolic adaptation in all three conditions and an elevated stress adaptation/tolerance specifically in the host environment. We conclude that asexual sporulation of M. graminicola during the late stage of plant infection occurs in a rich nutritional environment involving adaptation to stresses imposed in part by the presence of reactive oxygen species.
摘要
病原微生物必须成功适应宿主环境，获取营养并耐受免疫/防御反应。在转录组水平上研究宿主-病原体相互作用时，主要研究了宿主的反应。在这里，我们对真菌病原体在其宿主环境中进行孢子形成时进行了广泛的转录分析。叶点霉叶斑病是一种重要的小麦真菌病害，由子囊菌真菌叶点霉（无性型为麦球腔菌）引起。生成了包含 2563 个基因的 cDNA 微阵列，然后用于比较体外营养丰富和营养有限条件下以及植物感染后期体内真菌的营养和发育。获得的数据清楚地揭示了所有三种条件下的代谢适应，以及在宿主环境中特别升高的应激适应/耐受。我们得出结论，麦球腔菌在植物感染后期的无性孢子形成发生在富含营养的环境中，涉及对部分由活性氧引起的应激的适应。
