Gür S, Türk G, Demirci E, Yüce A, Sönmez M, Ozer S, Aksu Eh
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Fırat University, Başmakçı, Afyonkarahisar, Turkey.
Reprod Domest Anim. 2011 Apr;46(2):289-95. doi: 10.1111/j.1439-0531.2010.01660.x.
The aim of this study was to determine the changes in diameter of corpus luteum (CL), maternal progesterone (P) concentration, lipid peroxidation and non-enzymatic antioxidant levels along with enzymatic antioxidant activities in pregnant ewes bearing single and twin foetuses. The ewes were selected from healthy animals that were brought to the abattoir for slaughtering. The ewes were divided into three groups: Group 1 (non-pregnant, non-oestrous, n = 30), Group 2 (pregnant bearing a single foetus, n = 30) and Group 3 (pregnant bearing twin foetuses, n = 12) after they were slaughtered. Pregnant ewes were in the first half of the pregnancy. The diameter of CL and P concentration of pregnant ewes bearing a single foetus or twin foetuses were found higher than that found in non-pregnant ewes. Similarly, the P concentration of pregnant ewes bearing twin foetuses was higher than that found in pregnant ewes bearing a single foetus. Malondialdehyde (MDA) level in pregnant ewes bearing twin foetuses was higher than that found in both non-pregnant and pregnant ewes bearing a single foetus. The serum glutathione (GSH) level and glutathione-peroxidase (GSH-Px) activity of pregnant ewes bearing twin foetuses were found lower than that found in non-pregnant ewes. Additionally, the GSH-Px activity of pregnant ewes bearing twin foetuses was found lower than that found in pregnant ewes bearing a single foetus. No significant difference was found between pregnant ewes bearing female and male foetus with respect to diameter of CL, P concentration and oxidative stress parameters. There were significant positive correlations between foetal number (0, 1, 2) and diameter of CL, P concentration, MDA level, and between P concentration and diameter of CL, MDA level. However, significant negative correlations were found between foetal number (0, 1, 2) and GSH level, GSH-Px activity, and between P concentration and GSH-Px activity. In conclusion, the diameter of CL enlarges, P production increases and oxidant/antioxidant balance impairs because of the gestation stress in ewes during pregnancy.
本研究的目的是确定怀有单胎和双胎胎儿的怀孕母羊黄体（CL）直径、母体孕酮（P）浓度、脂质过氧化和非酶抗氧化剂水平以及酶促抗氧化剂活性的变化。这些母羊选自被送到屠宰场宰杀的健康动物。宰杀后，将母羊分为三组：第1组（未怀孕、未发情，n = 30）、第2组（怀有单胎胎儿的怀孕母羊，n = 30）和第3组（怀有双胎胎儿的怀孕母羊，n = 12）。怀孕母羊处于妊娠前半期。怀有单胎或双胎胎儿的怀孕母羊的CL直径和P浓度高于未怀孕母羊。同样，怀有双胎胎儿的怀孕母羊的P浓度高于怀有单胎胎儿的怀孕母羊。怀有双胎胎儿的怀孕母羊的丙二醛（MDA）水平高于未怀孕和怀有单胎胎儿的怀孕母羊。怀有双胎胎儿的怀孕母羊的血清谷胱甘肽（GSH）水平和谷胱甘肽过氧化物酶（GSH-Px）活性低于未怀孕母羊。此外，怀有双胎胎儿的怀孕母羊的GSH-Px活性低于怀有单胎胎儿的怀孕母羊。在怀有雌性和雄性胎儿的怀孕母羊之间，CL直径、P浓度和氧化应激参数方面未发现显著差异。胎儿数量（0、1、2）与CL直径、P浓度、MDA水平之间，以及P浓度与CL直径、MDA水平之间存在显著正相关。然而，胎儿数量（0、1、2）与GSH水平、GSH-Px活性之间，以及P浓度与GSH-Px活性之间存在显著负相关。总之，由于母羊在怀孕期间的妊娠应激，CL直径增大，P产生增加，氧化/抗氧化平衡受损。
