Güney Mehmet, Oral Baha, Mungan Tamer
Department of Obstetrics and Gynecology, Faculty of Medicine, Süleyman Demirel University, Isparta, Turkey.
Am J Perinatol. 2007 May;24(5):283-9. doi: 10.1055/s-2007-981429. Epub 2007 May 18.
Hyperemesis gravidarum (HEG) is a severe form of nausea and vomiting with unknown etiology. Recent studies have suggested that there might be an etiologic role for HELICOBACTER PYLORI (HP) in HEG. HP is a Gram-negative bacterium that colonizes the gastric mucosa, increases the generation of reactive oxygen species (ROS), and decreases plasma antioxidants such as ascorbic acid. The aim of this study was to investigate plasma ROS activities and antioxidant status, and their relationship with HP infection, in HEG womens. Twenty-five HEG women, 20 gestational age- and gravida-matched healthy pregnant controls, and 15 nonpregnant women were examined for specific immunoglobulin G (IgG) against HP, serum malondialdehyde (MDA) as a measure of lipid peroxidation, and activity of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CT). Compared with controls, the seropositivity of HP for IgG was significantly higher ( P < 0.01) in pregnancies with HEG. When three study groups (including both HP + and HP- patients) were compared with each other with respect to the study parameters, MDA concentration increased significantly in pregnant women when compared with control group (nonpregnant women; P < 0.01), as well as in HEG compared with healthy pregnant women ( P < 0.01). The SOD, GSH-Px, and CT activity were increased in the group of normal pregnant women versus the nonpregnant group ( P < 0.01), but decreased significantly in the group of HEG pregnant women ( P < 0.01). Serum MDA levels and SOD, GSH-Px, and CT activity were not affected by the seropositivity of HP for IgG in either group ( P > 0.05). The results of the present study suggest that HEG is an oxidative stress condition, as reflected by the increased ROS activity and decreased antioxidant status, regardless of HP infection.
妊娠剧吐（HEG）是一种病因不明的严重恶心和呕吐形式。最近的研究表明，幽门螺杆菌（HP）可能在妊娠剧吐中发挥病因学作用。HP是一种革兰氏阴性菌，可定植于胃黏膜，增加活性氧（ROS）的产生，并降低血浆抗氧化剂如抗坏血酸的水平。本研究的目的是调查妊娠剧吐女性的血浆ROS活性和抗氧化状态，以及它们与HP感染的关系。对25名妊娠剧吐女性、20名孕周和孕次匹配的健康孕妇对照组以及15名非孕妇进行了检测，以检测针对HP的特异性免疫球蛋白G（IgG）、作为脂质过氧化指标的血清丙二醛（MDA）以及抗氧化酶超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和过氧化氢酶（CT）的活性。与对照组相比，妊娠剧吐孕妇中HP IgG血清阳性率显著更高（P < 0.01）。当将三个研究组（包括HP阳性和HP阴性患者）在研究参数方面进行相互比较时，与对照组（非孕妇；P < 0.01）相比，孕妇的MDA浓度显著增加，与健康孕妇相比，妊娠剧吐孕妇的MDA浓度也显著增加（P < 0.01）。正常孕妇组与非孕妇组相比，SOD、GSH-Px和CT活性增加（P < 0.01），但妊娠剧吐孕妇组的这些活性显著降低（P < 0.01）。两组中HP IgG血清阳性率均未影响血清MDA水平以及SOD、GSH-Px和CT活性（P > 0.05）。本研究结果表明，无论是否感染HP，妊娠剧吐都是一种氧化应激状态，表现为ROS活性增加和抗氧化状态降低。
