Dept of Pathology, First Clinical Medical College, Shanxi Medical University, Taiyuan City, Shanxi, China.
J Exp Clin Cancer Res. 2010 Jun 16;29(1):71. doi: 10.1186/1756-9966-29-71.
SPARC (secreted protein, acidic and rich in cysteine) is closely related with the progress, invasion and metastasis of malignant tumor and angiogenesis.
Using human colon adenocarcinoma tissues (hereinafter referred to as colon cancer) and their corresponding non-diseased colon from 114 patients' biopsies, the expression of SPARC and vascular endothelial growth factor (VEGF) were investigated by immunohistochemistry staining to assessment the relationship between SPARC and VEGF, as well as their prognostic significance in patients. Evaluation of VEGF expression level with the same tissues was used to establish the antigenic profiles, and the marker of CD34 staining was used as an indicator of microvessel density (MVD).
SPARC expression was mainly in the stromal cells surrounding the colon cancer, and was significant difference in those tissues with the lymph node metastasis and differentiation degree of tumor. Expression of SPARC was significantly correlated with the expression of VEGF and MVD in colon cancer tissues. Patients with low or absence expressing SPARC had significantly worse overall survival and disease-free survival in a Single Factor Analysis; Cox Regression Analysis, SPARC emerged as an overall survival and disease-free survival independent prognostic factor for colon cancer.
The low expression or absence of stromal SPARC was an independent prognostic factor for poor prognosis of colon cancer. SPARC maybe involved in the regulation of anti-angiogenesis by which it may serve as a novel target for colon cancer treatment as well as a novel distinctive marker.
富含半胱氨酸的酸性分泌蛋白（SPARC）与恶性肿瘤的进展、侵袭和转移以及血管生成密切相关。
采用免疫组化染色法检测 114 例患者活检的人结肠腺癌组织（以下简称结肠癌）及其相应非病变结肠中 SPARC 和血管内皮生长因子（VEGF）的表达，评估 SPARC 与 VEGF 之间的关系，以及它们在患者预后中的意义。用相同组织评估 VEGF 表达水平，建立抗原谱，并用 CD34 染色标记物作为微血管密度（MVD）的指标。
SPARC 表达主要位于结肠癌周围的间质细胞中，在有淋巴结转移和肿瘤分化程度的组织中差异有统计学意义。SPARC 的表达与结肠癌组织中 VEGF 和 MVD 的表达显著相关。在单因素分析中，低表达或缺乏 SPARC 表达的患者总生存率和无病生存率明显较差；Cox 回归分析显示，SPARC 是结肠癌总生存率和无病生存率的独立预后因素。
基质 SPARC 的低表达或缺失是结肠癌预后不良的独立预后因素。SPARC 可能参与了抗血管生成的调节，因此它可能成为结肠癌治疗的新靶点和新的独特标志物。
