Department of General Surgery and Center of Minimal Invasive Gastrointestinal Surgery, Southwest Hospital, Third Military Medical University, Chongqing, China.
Eur Rev Med Pharmacol Sci. 2017 Oct;21(19):4311-4321.
To investigate tumor microenvironment of metastasis (TMEM) and the expression of SPARC (secreted protein acidic and rich in cysteine) in gastric cancer, and their relationships with hematogenous metastasis.
Twenty-six pairs of cases with gastric cancer were enrolled, in which there were 26 cases with distant organ metastases and 26 cases of gastric cancer without organ metastases as controls. TMEM (by double-stained immunohistochemistry) and the expression of SPARC were determined in twenty-six pairs of cases. In addition, we selected 48 patients to detect the expression of SPARC, VEGF (vascular endothelial growth factor), and evaluated TAMs (tumor associated macrophages), MVD (the microvessel density), MPI (microvessel pericyte coverage index), and TMEM in gastric cancer tissues by immunohistochemistry.
TMEM count was significantly higher in the metastatic gastric cancer tissues than that in non-metastatic cancer tissues in a case-control study (p<0.01). On the contrary, SPARC expression was lower in the metastatic gastric cancer tissues than that in non-metastatic cancer tissues. TMEM count, TAMs, and MVD were significantly correlated with invasion depth, histological type and TNM stage (p<0.05 or p<0.01). Expression of SPARC and VEGF were significantly correlated with tumor histological types, invasion depth, differentiation and lymph node metastasis of patients (p<0.05). SPARC and VEGF expression in stromal cells of gastric cancer tissues were significantly correlated with TAMs, MVD and MPI (p<0.05). In addition, SPARC expression was significantly inversely correlated with VEGF expression in gastric cancer tissues (p<0.05).
TMEM was detected in initial gastric cancer resection and closely correlated with hematogenous metastasis. Furthermore, SPARC may be involved in gastric cancer metastasis by effecting on tumor microenvironment.
探讨肿瘤转移微环境（TMEM）和富含半胱氨酸的酸性分泌蛋白（SPARC）在胃癌中的表达及其与血行转移的关系。
选取 26 对胃癌患者，其中 26 例有远处器官转移，26 例无器官转移作为对照。采用免疫组化双染法检测 26 对胃癌组织中 TMEM 和 SPARC 的表达情况。另外，选取 48 例患者，采用免疫组化法检测 SPARC、血管内皮生长因子（VEGF）的表达，评估肿瘤相关巨噬细胞（TAMs）、微血管密度（MVD）、微血管周细胞覆盖指数（MPI）和 TMEM 在胃癌组织中的表达。
病例对照研究显示，转移性胃癌组织中 TMEM 计数明显高于非转移性胃癌组织（p<0.01），而 SPARC 表达则明显低于非转移性胃癌组织。TMEM 计数、TAMs 和 MVD 与肿瘤浸润深度、组织学类型和 TNM 分期明显相关（p<0.05 或 p<0.01）。SPARC 和 VEGF 的表达与肿瘤组织学类型、浸润深度、分化程度和淋巴结转移明显相关（p<0.05）。胃癌组织基质细胞中 SPARC 和 VEGF 的表达与 TAMs、MVD 和 MPI 明显相关（p<0.05）。此外，胃癌组织中 SPARC 表达与 VEGF 表达呈明显负相关（p<0.05）。
在初始胃癌切除术中检测到 TMEM，与血行转移密切相关。此外，SPARC 可能通过影响肿瘤微环境参与胃癌转移。
