Department of Pathology, The First Clinical College of Harbin Medical University, Harbin 150001, China.
Pathol Oncol Res. 2012 Jul;18(3):697-702. doi: 10.1007/s12253-012-9497-9. Epub 2012 Jan 13.
We aimed to investigate the expression of SPARC (secreted protein, acidic and rich in cysteine) in gastric cancer and its relationship with tumor angiogenesis and cancer cells proliferation. Protein expression of SPARC, VEGF, CD34 and Ki-67 in 80 cases of gastric cancer and 30 cases of normal gastric tissue was evaluated by immunohistochemistry. CD34 staining was used as an indicator of microvessel density (MVD). Ki-67 labeling Index (LI) indicated cancer cells proliferation. Statistical analysis was used to investigate its relationship with clinical characteristics, tumor angiogenesis and cancer cells proliferation. SPARC expression was mainly in the stromal cells surrounding the gastric cancer cells, and was statistically significant differences between gastric cancer and normal gastric tissue (P < 0.05). Both the expression of SPARC and VEGF were related to differentiation degree, clinical stage, Lauren classification and lymph node metastasis (P < 0.05). Expression of SPARC was significantly negatively correlated with the expression of VEGF and MVD in gastric cancer tissues. Expression of SPARC was also negatively correlated with Ki-67-LI. Our findings suggest that both the expression of SPARC and VEGF are closed to tumor angiogenesis in gastric cancer, SPARC inhibited tumor angiogenesis but VEGF promoted tumor angiogenesis. SPARC also inhibited cells proliferation of gastric cancer.
研究富含半胱氨酸的酸性分泌蛋白（SPARC）在胃癌中的表达及其与肿瘤血管生成和癌细胞增殖的关系。采用免疫组织化学法检测 80 例胃癌组织和 30 例正常胃组织中 SPARC、VEGF、CD34 和 Ki-67 的蛋白表达，CD34 染色作为微血管密度（MVD）的指标，Ki-67 标记指数（LI）表示癌细胞增殖。统计分析其与临床特征、肿瘤血管生成和癌细胞增殖的关系。SPARC 表达主要位于胃癌细胞周围的间质细胞中，胃癌与正常胃组织之间差异有统计学意义（P<0.05）。SPARC 和 VEGF 的表达均与分化程度、临床分期、Lauren 分型和淋巴结转移有关（P<0.05）。胃癌组织中 SPARC 的表达与 VEGF 和 MVD 的表达呈显著负相关，SPARC 的表达与 Ki-67-LI 也呈负相关。研究结果表明，SPARC 和 VEGF 的表达均与胃癌中的肿瘤血管生成密切相关，SPARC 抑制肿瘤血管生成而 VEGF 促进肿瘤血管生成，SPARC 还抑制胃癌细胞增殖。
