Department of Biochemistry, University of Kerala, Kariavattom, Thiruvananthapuram 695581, Kerala, India.
Toxicol In Vitro. 2010 Sep;24(6):1662-9. doi: 10.1016/j.tiv.2010.05.021. Epub 2010 Jun 8.
Oxidative stress has long been recognized as an important mediator in the pathogenesis of cataract and the goal of this study was to determine the efficacy of isorhamnetin-3-glucoside (IR3G) in alleviating the toxicity induced by sodium selenite in in vitro culture condition. IR3G is the bioactive flavonoid isolated and characterized from the leaves of Cochlospermum religiosum. Enucleated rat lenses were maintained in organ culture containing M-199 medium alone (G-I), supplemented with 0.1 mM selenite (G-II) and selenite + 25 microg/ml IR3G (G-III). Treatment to G-III was from the second to fifth day while selenite administration to G-II & III was done on the third day. The antioxidant potential of the compound was assessed by Cu(2+) induced lipoprotein diene formation and superoxide scavenging assays. Morphological examination of the lenses also gave a supporting data. Antioxidant enzymes-superoxide dismutase (SOD), catalase and concentration of reduced glutathione (GSH) were significantly lower, while TBARS showed an increase in G-II than that in G-III and G-I lenses. Activity of Ca(2+)-ATPase was decreased and level of calcium was increased in G-II than G-III and G-I lenses. These data suggest that IR3G is able to significantly retard selenite cataract in vitro by virtue of its antioxidant property.
氧化应激一直被认为是白内障发病机制中的一个重要介质，本研究的目的是确定异鼠李素-3-葡萄糖苷（IR3G）在减轻体外培养条件下亚硒酸钠诱导的毒性方面的功效。IR3G 是从 Cochlospermum religiosum 的叶子中分离和鉴定出的生物活性类黄酮。将去核的大鼠晶状体在含有 M-199 培养基的器官培养物中维持（G-I），补充 0.1mM 亚硒酸钠（G-II）和亚硒酸钠+25μg/ml IR3G（G-III）。G-III 的处理从第二天到第五天进行，而 G-II 和 G-III 的亚硒酸钠给药在第三天进行。通过 Cu(2+)诱导的脂蛋白二烯形成和超氧化物清除测定评估化合物的抗氧化潜力。晶状体的形态学检查也提供了支持数据。抗氧化酶-超氧化物歧化酶（SOD）、过氧化氢酶和还原型谷胱甘肽（GSH）的浓度明显降低，而 TBARS 在 G-II 中比在 G-III 和 G-I 中增加。Ca(2+)-ATPase 的活性降低，G-II 中钙离子的水平比 G-III 和 G-I 中增加。这些数据表明，IR3G 因其抗氧化特性，能够显著延缓体外亚硒酸钠白内障的形成。
