Yağci Ramazan, Aydin Bahri, Erdurmuş Mesut, Karadağ Remzi, Gürel Ahmet, Durmuş Mustafa, Yiğitoğlu Ramazan
Fatih University, Medical School, Department of Ophthalmology, Ankara, Turkey.
Curr Eye Res. 2006 Oct;31(10):845-50. doi: 10.1080/02713680600899663.
To evaluate effects of melatonin on sodium selenite-induced cataract formation.
Twenty-three Sprague-Dawley rat pups were randomized into three groups. Group 1(n = 9), injected with selenite (s.c.) on postpartum day 10; group 2 (n = 7), injected with selenite (s.c.) on day 10 plus melatonin (i.p.) on days 8-15; group 3 (n = 7), saline-injected controls. Development of cataract was assessed weekly under a dissection microscope. Rat lenses and serums were analyzed for antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT); oxidative stress indicators xanthine oxidase (XO) and malondialdehyde (MDA), a marker of lipid peroxidation; and protein carbonyl (PC), a marker of protein oxidation.
Significant differences (p < 0.05) were seen in cataract development by the three groups. All rats developed dense nuclear cataract in group 1. Dense nuclear cataract was not observed in group 2: five of seven rats developed minor cataracts, while the other two had clear lenses. In control rats (group 3), all lenses remained clear. In selenite group (group 1), lens and serum levels of MDA, PC, and XO were significantly higher and levels of SOD and CAT were significantly lower than those in control group (p < 0.001). In selenite+melatonin group (group 2), lens and serum levels of MDA, PC, and XO significantly decreased and levels of SOD and CAT significantly increased when compared with selenite group.
Studies with the rat selenite cataract model strongly support the activity of melatonin as an endogenous antioxidant and anticataract agent.
评估褪黑素对亚硒酸钠诱导的白内障形成的影响。
将23只Sprague-Dawley幼鼠随机分为三组。第1组（n = 9），在出生后第10天皮下注射亚硒酸钠；第2组（n = 7），在第10天皮下注射亚硒酸钠，并在第8 - 15天腹腔注射褪黑素；第3组（n = 7），注射生理盐水作为对照。每周在解剖显微镜下评估白内障的发展情况。分析大鼠晶状体和血清中的抗氧化酶超氧化物歧化酶（SOD）和过氧化氢酶（CAT）；氧化应激指标黄嘌呤氧化酶（XO）和脂质过氧化标志物丙二醛（MDA）；以及蛋白质氧化标志物蛋白质羰基（PC）。
三组在白内障发展方面存在显著差异（p < 0.05）。第1组所有大鼠均发展为致密核性白内障。第2组未观察到致密核性白内障：7只大鼠中有5只发展为轻度白内障，另外2只晶状体清晰。在对照大鼠（第3组）中，所有晶状体均保持清晰。在亚硒酸钠组（第1组）中，晶状体和血清中的MDA、PC和XO水平显著高于对照组，而SOD和CAT水平显著低于对照组（p < 0.001）。与亚硒酸钠组相比，亚硒酸钠 + 褪黑素组（第2组）晶状体和血清中的MDA、PC和XO水平显著降低，SOD和CAT水平显著升高。
大鼠亚硒酸钠白内障模型研究有力地支持了褪黑素作为内源性抗氧化剂和抗白内障药物的活性。
