Department of Plastic Surgery, The University of Texas, MD Anderson Cancer Center, Houston, Texas, USA.
J Biomed Mater Res A. 2010 Aug;94(2):515-23. doi: 10.1002/jbm.a.32720.
Regenerative tissue engineering requires biomaterials that would mimic structure and composition of the extracellular matrix to facilitate cell infiltration, differentiation, and vascularization. Engineered scaffolds composed of natural biomaterials silk fibroin (SF) and chitosan (CS) blend were fabricated to achieve fibrillar nano-structures aligned in three-dimensions using the technique of dielectrophoresis. The effect of scaffold properties on adhesion and migration of human adipose-derived stem cells (hASC) and endothelial cells (HUVEC) was studied on SFCS (micro-structure, unaligned) and engineered SFCS (E-SFCS; nano-structure, aligned). E-SFCS constituted of a nano-featured fibrillar sheets, whereas SFCS sheets had a smooth morphology with unaligned micro-fibrillar extensions at the ends. Adhesion of hASC to either scaffolds occurred within 30 min and was higher than HUVEC adhesion. The percentage of moving cells and average speed was highest for hASC on SFCS scaffold as compared to hASC cocultured with HUVEC. HUVEC interactions with hASC appeared to slow the speed of hASC migration (in coculture) on both scaffolds. It is concluded that the guidance of cells for regenerative tissue engineering using SFCS scaffolds requires a fine balance between cell-cell interactions that affect the migration speed of cells and the surface characteristics that affects the overall adhesion and direction of migration.
再生组织工程需要生物材料来模拟细胞外基质的结构和组成，以促进细胞的渗透、分化和血管生成。本研究使用电介质电泳技术，制备了由天然生物材料丝素纤维（SF）和壳聚糖（CS）共混物组成的工程支架，以实现三维排列的纤维状纳米结构。研究了支架特性对人脂肪源性干细胞（hASC）和内皮细胞（HUVEC）黏附和迁移的影响，研究对象为 SFCS（微结构，无规）和工程化 SFCS（E-SFCS；纳米结构，排列）。E-SFCS 由具有纳米特征的纤维片构成，而 SFCS 片具有光滑的形态，末端有未对齐的微纤维延伸。hASC 与两种支架的黏附都在 30 分钟内发生，且黏附程度高于 HUVEC。与与 HUVEC 共培养的 hASC 相比，hASC 在 SFCS 支架上的迁移细胞比例和平均速度最高。HUVEC 与 hASC 的相互作用似乎会降低 hASC 在两种支架上（共培养时）的迁移速度。研究结论认为，使用 SFCS 支架进行再生组织工程的细胞导向需要在细胞-细胞相互作用和表面特性之间取得良好的平衡，以影响细胞的迁移速度和整体黏附及迁移方向。
