Conchello J A, Hansen E W
Appl Opt. 1990 Sep 10;29(26):3795-804. doi: 10.1364/AO.29.003795.
Significant improvement in the longitudinal (optical axis) resolution of a microscope has previously been obtained either by posterior digital processing of optical sections collected with a conventional microscope, or by collecting sections with a confocal scanning microscope. In this paper we report the feasibility of obtaining longitudinal resolution comparable to the lateral diffraction limit by posterior processing of confocal sections. A confocal through-focus image series was simulated numerically and restored by constrained iterative deconvolution and by maximum likelihood. Several typical imaging situations were simulated. The results support the possibility of achieving equal longitudinal and lateral resolution.
此前，通过对传统显微镜采集的光学切片进行后期数字处理，或者通过共聚焦扫描显微镜采集切片，已显著提高了显微镜的纵向（光轴）分辨率。在本文中，我们报告了通过对共聚焦切片进行后期处理来获得与横向衍射极限相当的纵向分辨率的可行性。通过数值模拟了一个共聚焦透焦图像序列，并通过约束迭代反卷积和最大似然法进行了恢复。模拟了几种典型的成像情况。结果支持了实现纵向和横向分辨率相等的可能性。
