College of Resources and Environment, Northwest A & F University, Yangling 712100, Shaanxi, People's Republic of China.
Antonie Van Leeuwenhoek. 2010 Oct;98(3):389-94. doi: 10.1007/s10482-010-9452-2. Epub 2010 Jun 23.
A novel pink-coloured, non-spore-forming, non-motile, Gram-negative bacterium, designated YIM 48858(T), is described by using a polyphasic approach. The strain can grow at pH 6.5-9 (optimum at pH 7) and 25-30 degrees C (optimum at 28 degrees C). NaCl is not required for its growth. Positive for oxidase and catalase. Urease activity, nitrate reduction, starch and Tween 80 tests are negative reaction. 16S rRNA gene sequence similarity studies showed that strain YIM 48858(T) is a member of the genus Rubellimicrobium, with similarities of 96.3, 95.7 and 95.5% to Rubellimicrobium mesophilum MSL-20(T), Rubellimicrobium aerolatum 5715S-9(T) and Rubellimicrobium thermophilum DSM 16684(T), respectively. Q-10 was the predominant respiratory ubiquinone as in the other members of the genus Rubellimicrobium. The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphoglycolipid, glycolipid and the major fatty acids were C18:1 omega7c, C16:0 and C10:0 3-OH, which are very different from the valid published species. The DNA G + C content was 67.7 mol%. Both phylogenetic and chemotaxonomic evidence supports that YIM 48858(T) is a novel species of the genus Rubellimicrobium, for which the name Rubellimicrobium roseum sp. nov. is proposed. The type strain is YIM 48858(T) (=CCTCC AA 208029(T) =KCTC 23202(T)).
一株新型粉红色、非孢子形成、非运动、革兰氏阴性细菌，被命名为 YIM 48858(T)，通过多相分类法进行描述。该菌株可在 pH6.5-9（最佳 pH7）和 25-30°C（最佳 28°C）下生长。其生长不需要 NaCl。氧化酶和过氧化氢酶阳性。脲酶活性、硝酸盐还原、淀粉和 Tween 80 试验均为阴性反应。16S rRNA 基因序列相似性研究表明，菌株 YIM 48858(T)是 Rubellimicrobium 属的一员，与 Rubellimicrobium mesophilum MSL-20(T)、Rubellimicrobium aerolatum 5715S-9(T)和 Rubellimicrobium thermophilum DSM 16684(T)的相似度分别为 96.3%、95.7%和 95.5%。Q-10 是与该属其他成员相同的主要呼吸泛醌。主要极性脂为双磷脂酰甘油、磷脂酰胆碱、磷糖脂、糖脂，主要脂肪酸为 C18:1ω7c、C16:0 和 C10:0 3-OH，与已发表的有效种非常不同。DNA G+C 含量为 67.7mol%。系统发育和化学分类学证据均支持 YIM 48858(T)是 Rubellimicrobium 属的一个新型种，因此提议将其命名为 Rubellimicrobium roseum sp. nov.。模式菌株为 YIM 48858(T)（=CCTCC AA 208029(T)=KCTC 23202(T)）。
