Yunnan Institute of Microbiology, Key Laboratory for Conservation and Utilization of Bio-Resource, School of Life Sciences, Yunnan University, Kunming, 650091, People's Republic of China.
Institute of Microbial Pharmaceuticals, Northeastern University, Shenyang, 110819, People's Republic of China.
Antonie Van Leeuwenhoek. 2019 Dec;112(12):1739-1745. doi: 10.1007/s10482-019-01304-5. Epub 2019 Jul 25.
A novel strain, YIM 131921, was isolated from a Physcia sp. lichen collected from the South Bank Forest of the Baltic Sea. The strain is Gram-negative, catalase positive and oxidase negative, strictly aerobic, asporogenous, non-motile and reddish brown in colour. The temperature and pH for growth were found to be 20-30 °C (optimum 28 °C) and pH 6.5-12.0 (optimum pH 7.0 ± 0.5). No growth was observed in the presence of NaCl. Based on 16S rRNA gene sequence similarity, strain YIM 131921 shares high similarities with Rubellimicrobium roseum YIM 48858 (98.3%), followed by Rubellimicrobium mesophilum MSL-20 (96.8%), Rubellimicrobium aerolatum 5715S-9 (96.1%) and Rubellimicrobium thermophilum DSM 16684 (96.0%). Phylogenetic trees showed YIM 131921 forms a cluster with type strains of the genus Rubellimicrobium. The predominant cellular fatty acids (> 20%) were identified as summed feature 8 (Cω7c) and C. Q-10 was found to be the predominant respiratory ubiquinone. The polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, glycolipid, phospholipids and an unidentified aminolipid. The DNA G + C content of the draft genome sequence is 66.6 mol%. Strain YIM 131921 showed an average nucleotide identity value of 80.3% and a digital DNA-DNA hybridizations value of 26.1% with the reference strain R. roseum YIM 48858 based on draft genome sequences. Based on comparative analyses of phenotypic, molecular, chemotaxonomic data and genomic comparisons, strain YIM 131921 is concluded to represent a novel species of the genus Rubellimicrobium, for which the name Rubellimicrobium rubrum sp. nov. is proposed. The type strain is YIM 131921 (= CGMCC 1.13958 = NBRC 114054 = KCTC 72461).
一株新的菌株 YIM 131921 从波罗的海南岸森林中的一种Physcia 地衣中分离出来。该菌株为革兰氏阴性、过氧化氢酶阳性、氧化酶阴性、严格需氧、无孢子、非运动、呈红棕色。其最适生长温度和 pH 值分别为 20-30°C（最适 28°C）和 pH 6.5-12.0（最适 pH 7.0±0.5）。在含有 NaCl 的环境中不能生长。基于 16S rRNA 基因序列相似性，菌株 YIM 131921 与 Rubellimicrobium roseum YIM 48858（98.3%）高度相似，其次是 Rubellimicrobium mesophilum MSL-20（96.8%）、Rubellimicrobium aerolatum 5715S-9（96.1%）和 Rubellimicrobium thermophilum DSM 16684（96.0%）。系统发育树显示 YIM 131921 与 Rubellimicrobium 属的模式菌株形成一个聚类。主要的细胞脂肪酸（>20%）被鉴定为总和特征 8（Cω7c），发现 Q-10 是主要的呼吸醌。极性脂类被鉴定为二磷脂酰甘油、磷脂酰甘油、磷脂酰胆碱、糖脂、磷脂和一种未鉴定的氨基脂类。该草案基因组序列的 DNA G+C 含量为 66.6 mol%。基于草案基因组序列，菌株 YIM 131921 与参考菌株 R. roseum YIM 48858 的平均核苷酸同一性值为 80.3%，数字 DNA-DNA 杂交值为 26.1%。基于表型、分子、化学分类学数据和基因组比较的综合分析，菌株 YIM 131921 被鉴定为 Rubellimicrobium 属的一个新种，命名为 Rubellimicrobium rubrum sp. nov.。其模式菌株为 YIM 131921（=CGMCC 1.13958=NBRC 114054=KCTC 72461）。
