逆转录病毒纯化：保留包膜糖蛋白并使感染性最大化的方法。
Retrovirus purification: method that conserves envelope glycoprotein and maximizes infectivity.
作者信息
McGrath M, Witte O, Pincus T, Weissman I L
出版信息
J Virol. 1978 Mar;25(3):923-7. doi: 10.1128/JVI.25.3.923-927.1978.
Abstract
A Sepharose 4B chromatographic method for purification of retroviruses is described which was less time consuming, increased purified virus yields, conserved viral glycoprotein, and increased recovery of biological infectivity in comparison with conventional sucrose gradient ultracentrifugation techniques.
摘要
描述了一种用于纯化逆转录病毒的琼脂糖4B色谱方法，与传统的蔗糖梯度超速离心技术相比，该方法耗时更少，提高了纯化病毒的产量，保留了病毒糖蛋白，并提高了生物感染性的回收率。
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