Ihle J N, Denny T P, Bolognesi D P
J Virol. 1976 Mar;17(3):727-36. doi: 10.1128/JVI.17.3.727-736.1976.
The major envelope glycoprotein (gp71) from AKR murine leukemia virus (MuLV) was purified and its serological reactivity with heterologous and autogenous immune mouse sera was examined. Homologous and interspecies competition radioimmunoassays using antisera to Rauscher-MulV gp69/71 or Friend-MuLV gp71 or antisera to feline leukemia virus to precipitate 125I-labeled gp71 from various MuLV showed that distinct differences exist between Rauscher- or Friend-MuLV and AKR-MuLV glycoproteins. Characteristically the AKR-MuLV gp71, in contrast to FLV or RLV gp71, does not compete fully in homologous or interspecies radioimmunoassays with iodinated Friend of Rauscher glycoproteins. Purified 125I-labeled AKR-MuLV gp71, in contrast to the Rauscher- or Friend-MuLV glycoproteins, reacts with normal (autogenous immune) mouse sera in direct radioimmune precipitation assays. Competition experiments further demonstrate that this is a predominant immunological reactivity of normal mouse sera which had previously been detected by radioimmune precipitation assay against intact virions.
从AKR小鼠白血病病毒（MuLV）中纯化出主要包膜糖蛋白（gp71），并检测其与异种和自身免疫小鼠血清的血清学反应性。使用抗劳斯氏肉瘤病毒-MuLV gp69/71或弗瑞德氏白血病病毒-MuLV gp71的抗血清或抗猫白血病病毒的抗血清进行同源和种间竞争放射免疫测定，以从各种MuLV中沉淀出125I标记的gp71，结果表明劳斯氏肉瘤病毒或弗瑞德氏白血病病毒与AKR-MuLV糖蛋白之间存在明显差异。与弗氏白血病病毒（FLV）或罗氏白血病病毒（RLV）的gp71不同，AKR-MuLV gp71的特征在于，在同源或种间放射免疫测定中，它不能与碘化的劳斯氏肉瘤病毒或弗瑞德氏白血病病毒糖蛋白完全竞争。与劳斯氏肉瘤病毒或弗瑞德氏白血病病毒糖蛋白不同，纯化的125I标记的AKR-MuLV gp71在直接放射免疫沉淀试验中能与正常（自身免疫）小鼠血清发生反应。竞争实验进一步证明，这是正常小鼠血清的主要免疫反应性，之前通过针对完整病毒粒子的放射免疫沉淀试验已检测到这种反应性。
