在产生性感染细胞中的传染性灵长类C型病毒前病毒DNA。
Infectious primate type C virus proviral DNA in productively infected cells.
作者信息
Markham P D, Gallo R C
出版信息
J Virol. 1978 Mar;25(3):936-9. doi: 10.1128/JVI.25.3.936-939.1978.
Abstract
Cell lines competent to infection by DNA from cultures chronically infected by type C viruses of the simian sarcoma virus and baboon endogenous virus groups were identified. Significant differences were observed in the relative susceptibility of some cell lines to infection by a given proviral DNA. Practical applications of transfection techniques for the separation of viruses from dually infected cultures and to free virus stocks from mycoplasmal contamination are described.
摘要
鉴定出了能够被猿猴肉瘤病毒和狒狒内源性病毒组的C型病毒长期感染的培养物中的DNA感染的细胞系。观察到某些细胞系对给定前病毒DNA感染的相对易感性存在显著差异。描述了转染技术在从双重感染培养物中分离病毒以及从支原体污染中净化病毒储备方面的实际应用。
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Isolation of infectious xenotropic mouse type C virus by transfection of a heterologous cell with DNA from a transformed mouse cell.通过用来自转化小鼠细胞的DNA转染异源细胞来分离感染性嗜异性小鼠C型病毒。
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