Robbins K C, Hill R L, Aaronson S A
J Virol. 1982 Feb;41(2):721-5. doi: 10.1128/JVI.41.2.721-725.1982.
We sought to identify the species of origin of the cell-derived (sis) sequences of simian sarcoma virus. A molecular clone comprised of sis DNA detected related nucleotide sequences at low copy numbers in normal cellular DNAs of species as diverse as humans and quail. The extent of hybridization and degree of base-pair matching with sis DNA were greatest with New World primate DNAs. The thermal denaturation curve midpoints of hybrids formed between sis and woolly monkey DNAs were indistinguishable from homologous sis DNA hybrids, establishing the woolly monkey (Lagothrix spp.) as the source of sis sequences. In comparative studies, sis was shown to be more conserved among mammalian species than unique-sequence woolly monkey cellular DNA. There was no detectable homology between sis and the cell-derived sequences of other fibroblast-transforming retroviruses. These findings indicate that sis is likely to be a unique onc gene among transforming retroviruses.
我们试图确定猿猴肉瘤病毒细胞衍生（sis）序列的起源物种。一个由sis DNA组成的分子克隆在人类和鹌鹑等多种物种的正常细胞DNA中以低拷贝数检测到相关核苷酸序列。与sis DNA的杂交程度和碱基对匹配程度在新世界灵长类动物DNA中最高。sis与绒毛猴DNA形成的杂交体的热变性曲线中点与同源sis DNA杂交体无法区分，确定绒毛猴（绒毛猴属）为sis序列的来源。在比较研究中，显示sis在哺乳动物物种中比独特序列的绒毛猴细胞DNA更保守。sis与其他成纤维细胞转化逆转录病毒的细胞衍生序列之间没有可检测到的同源性。这些发现表明，sis可能是转化逆转录病毒中一个独特的癌基因。
