Janezic Sandra, Rupnik Maja
Institute of Public Health Maribor, Centre for Microbiology, Maribor, Slovenia.
Methods Mol Biol. 2010;646:55-65. doi: 10.1007/978-1-60327-365-7_4.
Molecular typing methods for Clostridium difficile are based on gel electrophoresis of restriction fragments (endonuclease restriction analysis, REA; pulsed field gel electrophoresis PFGE; toxinotyping), PCR amplification (PCR ribotyping, arbitrarily primed PCR, multilocus variable-number tandem-repeat analysis MLVA), and sequence analysis (multilocus sequence typing MLST; slpA typing, tandem repeat sequence typing). We will describe two standard methods (PCR ribotyping predominantly used throughout Europe and PFGE which is predominantly used in North America) and will discuss the difficulties of inter-laboratory comparability and unification of typing nomenclature.
艰难梭菌的分子分型方法基于限制性片段的凝胶电泳（核酸内切酶限制性分析，REA；脉冲场凝胶电泳，PFGE；毒素分型）、PCR扩增（PCR核糖体分型、任意引物PCR、多位点可变数目串联重复分析，MLVA）以及序列分析（多位点序列分型，MLST；slpA分型、串联重复序列分型）。我们将描述两种标准方法（欧洲主要使用的PCR核糖体分型和北美主要使用的PFGE），并讨论实验室间可比性和分型命名统一方面的困难。
