[利用MUCAP试验检测水中的沙门氏菌]
[Detection of Salmonella in water by using MUCAP test].
作者信息
Zhang Wei, Sun Zongke, Lu Bo, Wang Youbin, Ding Pei, Zheng Ping, Chen Xiping
机构信息
Institute for Environment Hygiene and Health Related Product Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, China.
出版信息
Wei Sheng Yan Jiu. 2010 May;39(3):381-3.
OBJECTIVE
To study the sensitivity, specification, detection limit and the practical use of MUCAP method for the detection of Salmonella in water.
METHODS
A comprehensive method of pre-enrichment + selective cultivation + initial screening with MUCAP + oxidase test + serological test was applied to check Salmonella in water samples spiked with bacteria and environmental water samples.
RESULTS
the sensitivity of MUCAP method was 100%, the specification was 80.8%, and the detection limit was 1 cfu/100 ml.
CONCLUSION
MUCAP method can effectively identify Salmonella in water and the testing time was shortening from 72h to 48h.
目的
研究MUCAP法检测水中沙门氏菌的灵敏度、特异性、检测限及实际应用。
方法
采用预富集+选择性培养+MUCAP初筛+氧化酶试验+血清学试验的综合方法，对加菌水样和环境水样中的沙门氏菌进行检测。
结果
MUCAP法的灵敏度为100%，特异性为80.8%，检测限为1 cfu/100 ml。
结论
MUCAP法能有效鉴定水中的沙门氏菌，检测时间从72小时缩短至48小时。
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