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地表水中某些沙门氏菌检测方法的评估

An evaluation of certain Salmonella detection methods in surface water.

作者信息

Bernagozzi M, Sacchetti R, Polenta L

机构信息

Università degli Studi di Bologna, Facoltà di Medicina e Chirurgia, Dipartimento di Medicina e Sanità Pubblica.

出版信息

Zentralbl Hyg Umweltmed. 1996 Nov;199(1):84-90.

PMID:9353547
Abstract

Two different procedures were employed for detecting Salmonella spp. in environmental water samples: a rapid method (enrichment in Salmosyst Broth and plating in Rambach Agar-Merck) and a longer assay (pre-enrichment in Buffered Peptone Water, enrichment in Selenite-Cistyne Broth and plating in Brilliant Green Agar-Difco). The efficiency of microbiological tests was measured by the following criteria: recovery, sensitivity and specificity. The results analysed by the Kendall concordance coefficients demonstrated that the rapid method appeared more effective even if poorly specific.

摘要

采用两种不同的方法检测环境水样中的沙门氏菌属

一种快速方法(在Salmosyst肉汤中富集并接种于Rambach琼脂 - 默克公司产品)和一种较长时间的检测方法(在缓冲蛋白胨水中预富集,在亚硒酸盐 - 胱氨酸肉汤中富集并接种于亮绿琼脂 - Difco公司产品)。微生物检测的效率通过以下标准衡量:回收率、灵敏度和特异性。通过肯德尔和谐系数分析结果表明,快速方法似乎更有效,即使特异性较差。

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