Department of Biotechnology and Genetic Engineering, Medical University of Silesia, Sosnowiec, Poland.
Pol J Microbiol. 2010;59(1):11-20.
Bulking of activated sludge is a world-wide problem which negatively affects wastewater treatment efficiency. The most common reasons of bulking are bacterial community changes, especially excessive growth of filamentous bacteria (filamentous bulking) or excess of biopolymers on the surface of non-filamentous microbes (non-filamentous or Zoogleal bulking). Because of the complex nature of the bulking phenomenon finding a successful bulking control strategy remains a very important issue that awaits new options and advices. The REP-PCR fingerprinting method has been applied to distinguish a bacterial community in non-bulking and bulking activated sludge. The characteristic REP-PCR fingerprinting patterns were compared with each other in terms of the presence or absence of bands and in terms of measured integrated optical density (IOD) of the bands. The obtained fingerprinting patterns, using Ward's clustering method, have been analyzed to determine homology/similarity relations between specific non-bulking and bulking sludge sampling. The received clustering results were in high concordance with activated sludge typing which generally is done based on physicochemical sludge analysis. The proposed REP-PCR method and statistical analysis of fingerprinting patterns seems to be a simple, rapid and effective method revealing differences between populations in non-bulking and bulking activated sludge. It may be useful for routine activated sludge monitoring and may be helpful in the early detection of the bulking process.
活性污泥膨胀是一个全球性的问题，会对废水处理效率产生负面影响。膨胀的最常见原因是细菌群落的变化，特别是丝状菌的过度生长（丝状膨胀）或非丝状微生物表面的生物聚合物过多（非丝状或菌胶团膨胀）。由于膨胀现象的复杂性，寻找成功的膨胀控制策略仍然是一个非常重要的问题，需要新的选择和建议。REP-PCR 指纹图谱法已应用于区分非膨胀和膨胀活性污泥中的细菌群落。通过存在或不存在带以及带的测量积分光密度（IOD）来比较特征性 REP-PCR 指纹图谱模式。使用 Ward 聚类方法对获得的指纹图谱进行分析，以确定特定非膨胀和膨胀污泥采样之间的同源/相似关系。所得到的聚类结果与基于理化污泥分析的活性污泥分类高度一致。所提出的 REP-PCR 方法和指纹图谱的统计分析似乎是一种简单、快速和有效的方法，可以揭示非膨胀和膨胀活性污泥中种群之间的差异。它可用于常规活性污泥监测，并有助于早期检测膨胀过程。
